Deregulated microRNA and mRNA expression profiles in the peripheral blood of patients with Marfan syndrome

MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally. While acquired changes of miRNA and mRNA profiles in cancer have been extensively studied, little is known about expression changes of circulating miRNAs and messenger RNAs (mRNA) in monogenic constitutional anomali...

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Bibliographic Details
Published in:Journal of translational medicine 2018-03, Vol.16 (1), p.60-60, Article 60
Main Authors: Abu-Halima, Masood, Kahraman, Mustafa, Henn, Dominic, Rädle-Hurst, Tanja, Keller, Andreas, Abdul-Khaliq, Hashim, Meese, Eckart
Format: Article
Language:English
Subjects:
Blood cells
Care and treatment
Case-Control Studies
Cellular signal transduction
Cluster Analysis
Diagnosis
Female
Fibrillin
Gene expression
Gene Expression Profiling
Genetic aspects
Health aspects
Humans
Integration analysis
Male
Marfan syndrome
Marfan Syndrome - blood
Marfan Syndrome - genetics
MicroRNA
MicroRNAs - genetics
MicroRNAs - metabolism
mRNA
Open Reading Frames - genetics
Real-Time Polymerase Chain Reaction
Reproducibility of Results
RNA, Messenger - genetics
RNA, Messenger - metabolism
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Summary:MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally. While acquired changes of miRNA and mRNA profiles in cancer have been extensively studied, little is known about expression changes of circulating miRNAs and messenger RNAs (mRNA) in monogenic constitutional anomalies affecting several organ systems, like Marfan syndrome (MFS). We performed integrated miRNA and mRNA expression profiling in blood samples of Marfan patients in order to investigate deregulated miRNA and mRNA networks in these patients which could serve as potential diagnostic and prognostic tools for MFS therapy. MiRNA and mRNA expression profiles were determined in blood samples from MFS patients (n = 7) and from healthy volunteer controls (n = 7) by microarray analysis. Enrichment analyses of altered mRNA expression were identified using bioinformatic tools. A total of 28 miRNAs and 32 mRNAs were found to be significantly altered in MFS patients compared to controls (> 2.0-fold change, adjusted P 
ISSN:1479-5876
1479-5876
DOI:10.1186/s12967-018-1429-3