Protective Effects of Orange Sweet Pepper Juices Prepared by High-Speed Blender and Low-Speed Masticating Juicer against UVB-induced Skin Damage in SKH-1 Hairless Mice

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-09, Vol.27 (19), p.6394
• Main Authors: Truong, Van-Long, Rarison, Razanamanana. H. G, Jeong, Woo-Sik
• Format: Article
• Language: English
• Subjects: Aging; Alzheimer's disease; Analysis; Antioxidants; Beverages; Capsicum annuum; Carotenoids; Catalase; Collagen; Collagen (type I); Cyclooxygenase-2; Damage prevention; Deoxyribonucleic acid; DNA; DNA damage; Enzymes; Erythema; Extracellular matrix; Fibroblasts; Fruit juices; Gene regulation; Glutathione peroxidase; Hairless; High speed; high-speed blender; Households; Hyperplasia; Immunohistochemistry; Inflammation; Inflammatory response; Interleukin 17; Interleukin 23; Interleukins; Juices; Laboratories; Low speed; low-speed masticating juicer; Matrix metalloproteinase; Matrix metalloproteinases; Metalloproteinase; NF-κB protein; Nutrients; Oral administration; Oxidative stress; Peppers; Peroxidase; Polyphenols; Skin; skin health; Superoxide dismutase; sweet pepper juice; Tumorigenesis; Ultraviolet radiation; UVB radiation; Vegetables; Vitamin C
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27196394

Sweet pepper fruits (Capsicum annuum L.) contain various nutrients and phytochemicals that enhance human health and prevent the pathogenesis of certain diseases. Here, we report that oral administration of orange sweet pepper juices prepared by a high-speed blender and low-speed masticating juicer reduces UVB-induced skin damage in SKH-1 hairless mice. Sweet pepper juices reduced UVB-induced skin photoaging by the regulation of genes involved in dermal matrix production and maintenance such as collagen type I α 1 and matrix metalloproteinase-2, 3, 9. Administration of sweet pepper juices also restored total collagen levels in UVB-exposed mice. In addition, sweet pepper juices downregulated the expression of pro-inflammatory proteins such as cyclooxygenase-2, interleukin (IL)-1β, IL-17, and IL-23, which was likely via inhibiting the NF-κB pathway. Moreover, primary antioxidant enzymes in the skin were enhanced by oral supplementation of sweet pepper juices, as evidenced by increased expression of catalase, glutathione peroxidase, and superoxide dismutase-2. Immunohistochemical staining showed that sweet pepper juices reduced UVB-induced DNA damage by preventing 8-OHdG formation. These results suggest that sweet pepper juices may offer a protective effect against photoaging by inhibiting the breakdown of dermal matrix, inflammatory response, and DNA damage as well as enhancing antioxidant defense, which leads to an overall reduction in skin damage.