Redox proteomic study of Bacillus cereus thiol proteome during fermentative anaerobic growth

Bacillus cereus is a notorious foodborne pathogen, which can grow under anoxic conditions. Anoxic growth is supported by endogenous redox metabolism, for which the thiol redox proteome serves as an interface. Here, we studied the cysteine (Cys) proteome dynamics of B. cereus ATCC 14579 cells grown u...

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Bibliographic Details
Published in:BMC genomics 2021-09, Vol.22 (1), p.1-648, Article 648
Main Authors: Hamitouche, Fella, Gaillard, Jean-Charles, Schmitt, Philippe, Armengaud, Jean, Duport, Catherine, Dedieu, Luc
Format: Article
Language:English
Subjects:
Anaerobiosis
Anoxic conditions
Bacillus cereus
Bacteriology
Biochemistry, Molecular Biology
Carbohydrate metabolism
Carbohydrates
Cellular stress response
Datasets
Differential labeling strategy
Fermentation
Fermentative growth
Foodborne pathogens
Genetic aspects
Genomics
Homeostasis
Labeling
Life Sciences
Metabolic pathways
Metabolism
Microbial metabolism
Microbiological research
Microbiology and Parasitology
Peptides
Physiological aspects
Proteins
Proteomes
Proteomics
Redox potential
Thiol proteome
Thiols
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Summary:Bacillus cereus is a notorious foodborne pathogen, which can grow under anoxic conditions. Anoxic growth is supported by endogenous redox metabolism, for which the thiol redox proteome serves as an interface. Here, we studied the cysteine (Cys) proteome dynamics of B. cereus ATCC 14579 cells grown under fermentative anoxic conditions. We used a quantitative thiol trapping method combined with proteomics profiling. In total, we identified 153 reactive Cys residues in 117 proteins participating in various cellular processes and metabolic pathways, including translation, carbohydrate metabolism, and stress response. Of these reactive Cys, 72 were detected as reduced Cys. The B. cereus Cys proteome evolved during growth both in terms of the number of reduced Cys and the Cys-containing proteins identified, reflecting its growth-phase-dependence. Interestingly, the reduced status of the B. cereus thiol proteome increased during growth, concomitantly to the decrease of extracellular oxidoreduction potential. Taken together, our data show that the B. cereus Cys proteome during unstressed fermentative anaerobic growth is a dynamic entity and provide an important foundation for future redox proteomic studies in B. cereus and other organisms.
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-021-07962-y