A Transcriptional Analysis of the Genes Involved in the Ascorbic Acid Pathways Based on a Comparison of the Juice and Leaves of Navel and Anthocyanin-Rich Sweet Orange Varieties

Sweet oranges are an important source of ascorbic acid (AsA). In this study, the content of AsA in the juice and leaves of four orange clonal selections, different in terms of maturity time and the presence/absence of anthocyanins, was correlated with the transcription levels of the main genes invol...

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Bibliographic Details
Published in:Plants (Basel) 2021-06, Vol.10 (7), p.1291
Main Authors: Caruso, Paola, Russo, Maria Patrizia, Caruso, Marco, Guardo, Mario Di, Russo, Giuseppe, Fabroni, Simona, Timpanaro, Nicolina, Licciardello, Concetta
Format: Article
Language:English
Subjects:
Accumulation
Acids
Anthocyanins
Antioxidants
AsA metabolism
Ascorbic acid
Biosynthesis
Cell division
Citrus
citrus fruit
Citrus fruits
Citrus sinensis
Coronaviruses
Developmental stages
Enzymes
Fruit juices
Fruits
Galactose
Gene expression
Genes
Genotypes
Inositol
Juices
Metabolism
nonpigmented
pigmented
Ripening
Transcription
Vitamin C
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Summary:Sweet oranges are an important source of ascorbic acid (AsA). In this study, the content of AsA in the juice and leaves of four orange clonal selections, different in terms of maturity time and the presence/absence of anthocyanins, was correlated with the transcription levels of the main genes involved in the biosynthesis, recycling, and degradation pathways. Within each variety, differences in the above pathways and the AsA amount were found between the analysed tissues. Variations were also observed at different stages of fruit development and maturation. At the beginning of fruit development, AsA accumulation was attributable to the synergic action of l-galactose and Myo-inositol, while the l-gulose pathway was predominant between the end of fruit development and the beginning of ripening. In leaves, the l-galactose pathway appeared to play a major role in AsA accumulation, even though higher GalUr isoform expression suggests a synergistic contribution of both pathways in this tissue. In juice, the trend of the AsA content may be related to the decrease in the transcription levels of the GME, GDH, MyoOx, and GalUr12 genes. Newhall was the genotype that accumulated the most AsA. The difference between Newhall and the other varieties seems to be attributable to the GLDH, GalUr12, APX2, and DHAR3 genes.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10071291