The role of flavin mononucleotide (FMN) as a potentially clinically relevant biomarker to predict the quality of kidney grafts during hypothermic (oxygenated) machine perfusion

Hypothermic machine perfusion (HMP) provides preservation superior to cold storage and may allow for organ assessment prior to transplantation. Since flavin mononucleotide (FMN) in perfusate has been proposed as a biomarker of organ quality during HMP of donor livers, the aim of this study was to va...

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Bibliographic Details
Published in:PloS one 2023-06, Vol.18 (6), p.e0287713-e0287713
Main Authors: van de Leemkolk, Fenna E M, Lo Faro, M Letizia, Shaheed, Sadr, Mulvey, John F, Huurman, Volkert A L, Alwayn, Ian P J, Putter, Hein, Jochmans, Ina, Lindeman, Jan H N, Ploeg, Rutger J
Format: Article
Language:English
Subjects:
Biology and Life Sciences
Biomarkers
Biopsy
Chromatography
Chromatography, Liquid
Cold storage
Consent
Consortia
Context
Creatinine
Cryopreservation
Emission analysis
Excitation spectra
Flavin Mononucleotide
Fluorescence
Fluorescence spectroscopy
Graft rejection
Grafting
Hemodialysis
Ischemia
Kidney
Kidney transplantation
Kidney transplants
Kidneys
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medicine and Health Sciences
Organ Preservation - methods
Oxygenation
Perfusion
Perfusion - methods
Physical Sciences
Renal Dialysis
Research and Analysis Methods
Scientific imaging
Spectroscopy
Tandem Mass Spectrometry
Transplantation
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Summary:Hypothermic machine perfusion (HMP) provides preservation superior to cold storage and may allow for organ assessment prior to transplantation. Since flavin mononucleotide (FMN) in perfusate has been proposed as a biomarker of organ quality during HMP of donor livers, the aim of this study was to validate FMN as a biomarker for organ quality in the context of HMP preserved kidneys. Perfusate samples (n = 422) from the paired randomised controlled COPE-COMPARE-trial, comparing HMP with oxygenation (HMPO2) versus standard HMP in kidneys, were used. Fluorescence intensity (FI) was assessed using fluorescence spectroscopy (excitation 450nm; emission 500-600nm) and validated by fluorospectrophotometer and targeted liquid chromatography mass spectrometry (LC-MS/MS). Fluorescence intensity (FI)(ex450;em500-600) increased over time during machine perfusion in both groups (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0287713