Insights into the Role of Transcriptional Gene Silencing in Response to Herbicide-Treatments in Arabidopsis thaliana

Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether transcriptional gene silencing (TGS) and RNA-directed DNA Methylation (RdDM) pathways modulate resistance to commo...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-03, Vol.22 (7), p.3314
Main Authors: Markus, Catarine, Pecinka, Ales, Merotto, Jr, Aldo
Format: Article
Language:English
Subjects:
2,4-D
Adaptation
Arabidopsis thaliana
Binding sites
Biosynthesis
chromatin mutants
Demethylation
Deoxyribonucleic acid
DNA
DNA methylation
Epigenetics
Flavonoids
Flowers & plants
Gene silencing
Genes
Genomes
Glyphosate
Herbicide resistance
Herbicides
Hypersensitivity
imazethapyr
Mutants
Mutation
RNA polymerase
RNA-mediated interference
ROS1
Transcriptomics
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Summary:Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether transcriptional gene silencing (TGS) and RNA-directed DNA Methylation (RdDM) pathways modulate resistance to commonly applied herbicides. Using wild-type plants exposed to sublethal doses of glyphosate, imazethapyr, and 2,4-D, we found a partial loss of TGS and increased susceptibility to herbicides in six out of 11 tested TGS/RdDM mutants. Mutation in ( ), that plays an important role in DNA demethylation, leading to strongly increased susceptibility to all applied herbicides, and imazethapyr in particular. Transcriptomic analysis of the imazethapyr-treated wild type and plants revealed a relation of the herbicide upregulated genes to chemical stimulus, secondary metabolism, stress condition, flavonoid biosynthesis, and epigenetic processes. Hypersensitivity to imazethapyr of the flavonoid biosynthesis component ( ) mutant plants strongly suggests that ROS1-dependent accumulation of flavonoids is an important mechanism for herbicide stress response in . In summary, our study shows that herbicide treatment affects transcriptional gene silencing pathways and that misregulation of these pathways makes Arabidopsis plants more sensitive to herbicide treatment.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22073314