First Attempt to Couple Proteomics with the AhR Reporter Gene Bioassay in Soil Pollution Monitoring and Assessment

A topsoil sample obtained from a highly industrialized area (Taranto, Italy) was tested on the DR-CALUX cell line and the exposed cells processed with proteomic and bioinformatics analyses. The presence of polyhalogenated compounds in the topsoil extracts was confirmed by GC-MS/MS analysis. Proteomi...

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Bibliographic Details
Published in:Toxics (Basel) 2021-12, Vol.10 (1), p.9
Main Authors: Landi, Claudia, Liberatori, Giulia, Cotugno, Pietro, Sturba, Lucrezia, Vannuccini, Maria Luisa, Massari, Federica, Miniero, Daniela Valeria, Tursi, Angelo, Shaba, Enxhi, Behnisch, Peter A, Carleo, Alfonso, Di Giuseppe, Fabrizio, Angelucci, Stefania, Bini, Luca, Corsi, Ilaria
Format: Article
Language:English
Subjects:
Apoptosis
bioassay
Bioassays
Bioinformatics
Biological activity
Chemical analysis
Chemicals
Communication
contaminants of emerging concern
Dioxins
Environmental monitoring
Environmental risk
Epidemiology
Gene expression
Glycerol
Hydrocarbons
Industrial areas
Laboratories
Mass spectrometry
Pollution monitoring
polyhalogenated compounds
Proteins
Proteomics
Reporter gene
Risk assessment
Scientific imaging
Soil chemistry
Soil investigations
Soil pollution
Topsoil
Toxicity
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Summary:A topsoil sample obtained from a highly industrialized area (Taranto, Italy) was tested on the DR-CALUX cell line and the exposed cells processed with proteomic and bioinformatics analyses. The presence of polyhalogenated compounds in the topsoil extracts was confirmed by GC-MS/MS analysis. Proteomic analysis of the cells exposed to the topsoil extracts identified 43 differential proteins. Enrichment analysis highlighted biological processes, such as the cellular response to a chemical stimulus, stress, and inorganic substances; regulation of translation; regulation of apoptotic process; and the response to organonitrogen compounds in light of particular drugs and compounds, extrapolated by bioinformatics all linked to the identified protein modifications. Our results confirm and reflect the complex epidemiological situation occurring among Taranto inhabitants and underline the need to further investigate the presence and sources of inferred chemicals in soils. The combination of bioassays and proteomics reveals a more complex scenario of chemicals able to affect cellular pathways and leading to toxicities rather than those identified by only bioassays and related chemical analysis. This combined approach turns out to be a promising tool for soil risk assessment and deserves further investigation and developments for soil monitoring and risk assessment.
ISSN:2305-6304
2305-6304
DOI:10.3390/toxics10010009