The effect of probiotics on immunogenicity of spermatozoa in couples suffering from recurrent spontaneous abortion

• Published in: BMC immunology 2022-06, Vol.23 (1), p.1-32, Article 32
• Main Authors: Rafiee, Mitra, Sereshki, Nasrin, Alipour, Razieh, Ahmadipanah, Vahid, Pashoutan Sarvar, Davod, Wilkinson, David
• Format: Article
• Language: English
• Subjects: Antibodies; Antigenicity; Antigens; APCA; Cytotoxicity; Enzyme-linked immunosorbent assay; Evaluation; Flow cytometry; Histocompatibility antigen HLA; HLA class I & II; Immune system; Immunogenetics; Immunogenicity; Immunoglobulin G; Leukocytes (mononuclear); Ligands; Methods; Microbiota; Miscarriage; Peripheral blood mononuclear cells; Physiological aspects; Pregnancy complications; Probiotic; Probiotics; Recurrent spontaneous abortion (RSA); Reproductive system; Sperm; Spermatozoa; Vaccines; Vagina; Womens health
• ISSN: 1471-2172; 1471-2172
• DOI: 10.1186/s12865-022-00506-3

Impaired spermatozoa immunogenicity can result in pregnancy complications such as recurrent spontaneous abortion (RSA). Given that spermatozoa contact with microbiota, it is possible that inappropriate microbiota composition in the reproductive tract could result in the alteration of spermatozoa antigenicity. Probiotics, as a representative of microbiota, may therefore have a beneficial effect on this altered immunogenicity. The objective of this study was to determine the effect of probiotics on spermatozoa immunogenicity. Twenty-five fertile couples and twenty-five RSA couples were included in this study. Spermatozoa were purified and treated with probiotics. Untreated and probiotic treated spermatozoa were evaluated for human leukocyte antigen (HLA) class I & II expression by flow cytometry. Untreated and probiotic treated spermatozoa were also cocultured with the wife's peripheral blood mononuclear cells (PBMC) for 12 days. Then, the supernatant was assessed for IgG and APCA by enzyme-linked immunosorbent assay (ELISA) and complement-dependent cytotoxicity (CDC) assay respectively. Probiotic treatment of spermatozoa leads to an increase of HLA class I & II expression in both the fertile and RSA groups. The probiotic treatment resulted in a decrease in both IgG and APCA in the fertile group, but an increase in both IgG and APCA in the RSA group. The results of this study suggest that a supplementary probiotic treatment may be useful in couples suffering from RSA with an immunologic cause, because it improves disturbed HLA expression on spermatozoa and improves disturbed APCA and IgG production in the presence of spermatozoa.