Electroanalytical analysis of phenol oxidation using bacteria immobilized by a polycaprolactone coating on the copper electrode surface

• Published in: Scientific reports 2024-06, Vol.14 (1), p.13136-12
• Main Authors: Moutcine, Abdelaziz, Laghlimi, Charaf, Ziat, Younes, Isaad, Jalal, El Bahraoui, Soumia, Chtaini, Abdelilah
• Format: Article
• Language: English
• Subjects: 639/638/11; 639/638/161; Bacteria; Biosensors; Contact angle; Copper; Cyclic voltammetry; Electrochemical impedance spectroscopy; Electrochemistry; Electrodes; Electrolytes; Electrons; Experiments; Graphene; Humanities and Social Sciences; Modified copper electrode; multidisciplinary; Oxidation; Phenol oxidation; Phenols; Polycaprolactone; Polymers; Science; Science (multidisciplinary); Spectroscopy; Spectrum analysis; Square wave voltammetry; Toxicity; Voltammetry
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-58281-7

The copper electrode modified by bacteria immobilised by a polycaprolactone film was successfully developed by electropolymerisation for the purpose of determining the presence of phenol. Electrochemical techniques such as square-wave voltammetry (SWV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the electrochemical properties of the Cu-polymer/bacteria electrode. The results show that the intensity of the phenol oxidation peak increases with concentration, allowing us to obtain good analytical results with DL of 2.156 × 10 –7 M and QL which is 7.2 × 10 –7 M , confirming that the biosensor has excellent electroanalytical activity for phenol oxidation, with good stability and a wide linear range. Our electrode is based on a easily available and inexpensive material, as well as on its simple preparation, which has demonstrated high performance for phenol.