PTPN9 induces cell apoptosis by mitigating the activation of Stat3 and acts as a tumor suppressor in colorectal cancer

Accumulating evidence has shown that protein tyrosine phosphatases (PTPs) are involved in regulating the transduction of many signaling pathways and play important roles in modulating the progression of some cancers, but the functions of PTPs in cancers have not been well elucidated until now. Here,...

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Bibliographic Details
Published in:Cancer management and research 2019-01, Vol.11, p.1309-1319
Main Authors: Wang, Dawei, Cheng, Zhuoxin, Zhao, Ming, Jiao, Chengbin, Meng, Qinghui, Pan, Huayang, Xie, Yu, Li, Long, Zhu, Yexing, Wang, Wei, Qu, Chunlei, Liang, Deshen
Format: Article
Language:English
Subjects:
Analysis
Apoptosis
Breast cancer
Cancer therapies
Cell cycle
Cell growth
cell survival
Cervical cancer
Colorectal cancer
Development and progression
Gastric cancer
Growth factors
Health aspects
Hospitals
Laboratories
Liver cancer
Metastasis
MicroRNAs
Original Research
Phosphatase
Phosphatases
Proteins
PTPMeg2
PTPN9
Scientific equipment and supplies industry
Stat3
Surgery
Tumors
Tyrosine
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Summary:Accumulating evidence has shown that protein tyrosine phosphatases (PTPs) are involved in regulating the transduction of many signaling pathways and play important roles in modulating the progression of some cancers, but the functions of PTPs in cancers have not been well elucidated until now. Here, we aimed to identify the roles of protein tyrosine phosphatase nonreceptor type 9 (PTPN9), a cytoplasmic PTP, in the development of colorectal cancer and elucidate the regulatory mechanism involved. Cell viability assessment, colony formation assay, caspase-3 and caspase-9 activity assay, real-time PCR, and Western blot analysis were applied. Our results showed that PTPN9 expression was frequently downregulated in colorectal cancer tissues compared with adjacent normal tissues. Overexpression of PTPN9 mitigated cell growth and colony formation and induced cell apoptosis in colorectal cancer. Conversely, PTPN9 knockdown promoted cell growth and survival. Moreover, PTPN9 negatively regulated the activation of Stat3 and depressed its nuclear translocation in colorectal cancer. The effects of PTPN9 knockdown on cell apoptosis were attenuated by inhibition of the Stat3 pathway. These results indicate that PTPN9 inhibits cell growth and survival by repressing the activation of Stat3 in colorectal cancer, which suggests an important underlying mechanism of regulating cell growth and provides a novel candidate therapeutic target for colorectal cancer.
ISSN:1179-1322
1179-1322
DOI:10.2147/CMAR.S187001