Metformin-Encapsulated Liposome Delivery System: An Effective Treatment Approach against Breast Cancer

This study aimed at developing metformin hydrochloride (Met) encapsulated liposomal vesicles for enhanced therapeutic outcomes at reduced doses against breast cancer. Liposomal Met was prepared using thin-film hydration through various loading methods; passive loading, active loading, and drug-loade...

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Bibliographic Details
Published in:Pharmaceutics 2019-10, Vol.11 (11), p.559
Main Authors: Shukla, Snehal K, Kulkarni, Nishant S, Chan, Amanda, Parvathaneni, Vineela, Farrales, Pamela, Muth, Aaron, Gupta, Vivek
Format: Article
Language:English
Subjects:
3d spheroids
ampk activation
Breast cancer
Cancer therapies
Chemotherapy
Drug dosages
Entrapment
Homeostasis
Hydration
Kinases
Lipids
liposomes
metformin
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Summary:This study aimed at developing metformin hydrochloride (Met) encapsulated liposomal vesicles for enhanced therapeutic outcomes at reduced doses against breast cancer. Liposomal Met was prepared using thin-film hydration through various loading methods; passive loading, active loading, and drug-loaded lipid film. The drug-loaded film method exhibited maximum entrapment efficiency (~65%) as compared to active loading (~25%) and passive loading (~5%) prepared Met-loaded liposomes. The therapeutic efficacy of these optimized liposomes was evaluated for cellular uptake, cytotoxicity, inhibition of metastatic activity, and apoptosis-inducing activity. Results demonstrated significantly superior activity of positively charged liposomes resulting in reduced IC values, minimal cell migration activity, reduced colony formation, and profound apoptosis-induced activity in breast cancer cells as compared to Met. The anti-tumor activity was investigated using a clinically relevant in vitro tumor simulation model, which confirmed enhanced anti-tumorigenic property of liposomal Met over Met itself. To the authors' knowledge, this is the first report of Met-loaded liposomes for improving the efficacy and therapeutic effect of Met against breast cancer. With the results obtained, it can be speculated that liposomal encapsulation of metformin offers a potentially promising and convenient approach for enhanced efficacy and bioavailability in breast cancer treatment.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics11110559