BUB-1 targets PP2A:B56 to regulate chromosome congression during meiosis I in C. elegans oocytes

Protein Phosphatase 2A (PP2A) is a heterotrimer composed of scaffolding (A), catalytic (C), and regulatory (B) subunits. PP2A complexes with B56 subunits are targeted by Shugoshin and BUBR1 to protect centromeric cohesion and stabilise kinetochore-microtubule attachments in yeast and mouse meiosis....

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Bibliographic Details
Published in:eLife 2020-12, Vol.9
Main Authors: Bel Borja, Laura, Soubigou, Flavie, Taylor, Samuel J P, Fraguas Bringas, Conchita, Budrewicz, Jacqueline, Lara-Gonzalez, Pablo, Sorensen Turpin, Christopher G, Bembenek, Joshua N, Cheerambathur, Dhanya K, Pelisch, Federico
Format: Article
Language:English
Subjects:
Animals
B56
Bub1
Caenorhabditis elegans - metabolism
Caenorhabditis elegans - physiology
Caenorhabditis elegans Proteins - metabolism
Caenorhabditis elegans Proteins - physiology
Cell Biology
Chromosome Segregation
Chromosomes
Chromosomes - physiology
meiosis
Meiosis - physiology
Oocytes - metabolism
Oocytes - physiology
Phosphatases
PP2A
Protein Phosphatase 2 - metabolism
Protein Phosphatase 2 - physiology
Protein Serine-Threonine Kinases - metabolism
Protein Serine-Threonine Kinases - physiology
SLiM
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Summary:Protein Phosphatase 2A (PP2A) is a heterotrimer composed of scaffolding (A), catalytic (C), and regulatory (B) subunits. PP2A complexes with B56 subunits are targeted by Shugoshin and BUBR1 to protect centromeric cohesion and stabilise kinetochore-microtubule attachments in yeast and mouse meiosis. In , the closest BUBR1 orthologue lacks the B56-interaction domain and Shugoshin is not required for meiotic segregation. Therefore, the role of PP2A in female meiosis is unknown. We report that PP2A is essential for meiotic spindle assembly and chromosome dynamics during female meiosis. BUB-1 is the main chromosome-targeting factor for B56 subunits during prometaphase I. BUB-1 recruits PP2A:B56 to the chromosomes via a newly identified LxxIxE motif in a phosphorylation-dependent manner, and this recruitment is important for proper chromosome congression. Our results highlight a novel mechanism for B56 recruitment, essential for recruiting a pool of PP2A involved in chromosome congression during meiosis I.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.65307