Antibiotic-resistant characteristics and horizontal gene transfer ability analysis of extended-spectrum β-lactamase-producing Escherichia coli isolated from giant pandas

• Published in: Frontiers in veterinary science 2024-07, Vol.11, p.1394814
• Main Authors: Liu, Haifeng, Fan, Siping, Zhang, Xiaoli, Yuan, Yu, Zhong, Wenhao, Wang, Liqin, Wang, Chengdong, Zhou, Ziyao, Zhang, Shaqiu, Geng, Yi, Peng, Guangneng, Wang, Ya, Zhang, Kun, Yan, Qigui, Luo, Yan, Shi, Keyun, Zhong, Zhijun
• Format: Article
• Language: English
• Subjects: antibiotic resistance gene; Escherichia coli; extended-spectrum β-lactamase; giant panda; horizontal gene transfer; virulence-associated gen
• ISSN: 2297-1769; 2297-1769
• DOI: 10.3389/fvets.2024.1394814

Extended-spectrum β-lactamase (ESBL)-producing (ESBL-EC) is regarded as one of the most important priority pathogens within the One Health interface. However, few studies have investigated the occurrence of ESBL-EC in giant pandas, along with their antibiotic-resistant characteristics and horizontal gene transfer abilities. In this study, we successfully identified 12 ESBL-EC strains (8.33%, 12/144) out of 144 strains which isolated from giant pandas. We further detected antibiotic resistance genes (ARGs), virulence-associated genes (VAGs) and mobile genetic elements (MGEs) among the 12 ESBL-EC strains, and the results showed that 13 ARGs and 11 VAGs were detected, of which (100.00%, 12/12, with 5 variants observed) and (83.33%, 10/12) were the most prevalent, respectively. And (66.67%, 8/12) and (66.67%, 8/12) were the predominant MGEs. Furthermore, horizontal gene transfer ability analysis of the 12 ESBL-EC showed that all genes could be transferred by conjugative plasmids, indicating high horizontal gene transfer ability. In addition, ARGs of and , VAGs of , and , MGEs of and were all found to be co-transferred with . Phylogenetic analysis clustered these ESBL-EC strains into group B2 (75.00%, 9/12), D (16.67%, 2/12), and B1 (8.33%, 1/12), and 10 sequence types (STs) were identified among 12 ESBL-EC (including ST48, ST127, ST206, ST354, ST648, ST1706, and four new STs). Our present study showed that ESBL-EC strains from captive giant pandas are reservoirs of ARGs, VAGs and MGEs that can co-transfer with via plasmids. Transmissible ESBL-EC strains with high diversity of resistance and virulence elements are a potential threat to humans, animals and surrounding environment.