Purshia plicata Triggers and Regulates Proteins Related to Apoptosis in HeLa Cancer Cells

Cervical cancer represents a public health problem, develops resistance to traditional therapies and cost-of-treatment is high. These disadvantages have led to the search for alternative bioactive-compound-based therapies. Said bioactive compounds include phenolic compounds, flavonoids, and tannins....

Full description

Saved in:
Bibliographic Details
Published in:Plants (Basel) 2021-11, Vol.10 (12), p.2559
Main Authors: Álvarez-Ortiz, Patricia, Ascacio-Valdés, Juan, Vera-Reyes, Ileana, Esparza-González, Cecilia, Rodríguez-Herrera, Raúl, Salinas-Santander, Mauricio, Del Ángel-Martínez, Mayela, Morlett-Chávez, Antonio
Format: Article
Language:English
Subjects:
Apoptosis
bio-compounds
Bioactive compounds
Biological activity
Cancer
Cell cycle
Cell viability
Cervical cancer
Cervix
Deregulation
Drug resistance
Electric fields
Fibroblasts
Flavonoids
HeLa
High performance liquid chromatography
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medical research
P. plicata
Peptides
Phenolic compounds
Phenols
Phytochemicals
Polyphenols
Protein expression
Proteins
Public health
Spectroscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cervical cancer represents a public health problem, develops resistance to traditional therapies and cost-of-treatment is high. These disadvantages have led to the search for alternative bioactive-compound-based therapies. Said bioactive compounds include phenolic compounds, flavonoids, and tannins. The present study aimed to evaluate the therapeutic effect of a extract on the HeLa cell line. Viability and apoptosis assays were run on the two cell lines treated with the extract. The peptides, up- and down-expressed in both cell lines, were identified by PDQuest analysis software and high-performance liquid chromatography/mass spectrometry/mass spectrometry (HPLC/MS/MS). Our results show that a 500 mg/L treatment deregulated cell viability, with different apoptotic morphologies observed which are associated with the presence of bio-compounds, which up- and down-regulated the peptides. In conclusion, regulates proteins associated with apoptosis in HeLa cancer cells.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10122559