An unusual overrepresentation of genetic factors related to iron homeostasis in the genome of the fluorescent Pseudomonas sp. ABC1

Summary Members of the genus Pseudomonas inhabit diverse environments, such as soil, water, plants and humans. The variability of habitats is reflected in the diversity of the structure and composition of their genomes. This cosmopolitan bacterial genus includes species of biotechnological, medical...

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Published in:Microbial biotechnology 2021-05, Vol.14 (3), p.1060-1072
Main Authors: Valenzuela‐Heredia, Daniel, Henríquez‐Castillo, Carlos, Donoso, Raúl, Lavín, Paris, Ringel, Michael T., Brüser, Thomas, Campos, José Luis
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Azotobacter
Biosynthesis
Biotechnology
Fluorescence
Gene regulation
Genes
Genetic factors
Genomes
Genomics
Homeostasis
Humans
Iron
Peptides
Phylogenetics
Plant bacterial diseases
Plant growth
Pseudomonas
Pseudomonas - genetics
Pyoverdine
RNA polymerase
Siderophores
Signal transduction
Soil analysis
Soil water
Soils
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Summary:Summary Members of the genus Pseudomonas inhabit diverse environments, such as soil, water, plants and humans. The variability of habitats is reflected in the diversity of the structure and composition of their genomes. This cosmopolitan bacterial genus includes species of biotechnological, medical and environmental importance. In this study, we report on the most relevant genomic characteristics of Pseudomonas sp. strain ABC1, a siderophore‐producing fluorescent strain recently isolated from soil. Phylogenomic analyses revealed that this strain corresponds to a novel species forming a sister clade of the recently proposed Pseudomonas kirkiae. The genomic information reveals an overrepresented repertoire of mechanisms to hoard iron when compared to related strains, including a high representation of fecI‐fecR family genes related to iron regulation and acquisition. The genome of the Pseudomonas sp. ABC1 contains the genes for non‐ribosomal peptide synthetases (NRPSs) of a novel putative Azotobacter‐related pyoverdine‐type siderophore, a yersiniabactin‐type siderophore and an antimicrobial betalactone; the last two are found only in a limited number of Pseudomonas genomes. Strain ABC1 can produce siderophores in a low‐cost medium, and the supernatants from cultures of this strain promote plant growth, highlighting their biotechnological potential as a sustainable industrial microorganism. ‘Pseudomonas chilensis’ strain ABC1 was fully sequenced. About 5% of the ABC1 genome is devoted to iron acquisition, including 37 ECF sigma factors. Strain ABC1 produces pyoverdine and has the genes for the production of yersiniabactin.
ISSN:1751-7915
1751-7915
DOI:10.1111/1751-7915.13753