Association between PPARG genetic polymorphisms and ischemic stroke risk in a northern Chinese Han population: a case-control study

• Published in: Neural regeneration research 2019-11, Vol.14 (11), p.1986-1993
• Main Authors: Wang, Yan-Zhe, Zhang, He-Yu, Liu, Fang, Li, Lei, Deng, Shu-Min, He, Zhi-Yi
• Format: Article
• Language: English
• Subjects: Age; Alcohol use; Analysis; Atherosclerosis; Body mass index; Deoxyribonucleic acid; Diabetes; Disease; Disease susceptibility; DNA; Genes; Genetic aspects; Genetic polymorphisms; Genotype & phenotype; Haplotypes; Hypertension; Ischemia; nerve regeneration; stroke; cerebral ischemia; ischemic stroke; peroxisome proliferator-activated receptor γ single-nucleotide polymorphism; haplotype analysis; interaction; case-control study; Chinese Han population; neural regeneration; Pathogenesis; Patients; Risk assessment; Risk factors; Single nucleotide polymorphisms; Stroke; Type 2 diabetes
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.259621

Two common polymorphisms of the peroxisome proliferator-activated receptor gamma (PPARG) gene, rs1801282 and rs3856806, may be important candidate gene loci affecting the susceptibility to ischemic stroke. This case-control study sought to identify the relationship between these two single-nucleotide polymorphisms and ischemic stroke risk in a northern Chinese Han population. A total of 910 ischemic stroke participants were recruited from the First Hospital of China Medical University, Shenyang, China as a case group, of whom 895 completed the study. The 883 healthy controls were recruited from the Health Check Center of the First Hospital of China Medical University, Shenyang, China. All participants or family members provided informed consent. The study protocol was approved by the Ethics Committee of the First Hospital of China Medical University, China on February 20, 2012 (approval No. 2012-38-1). The protocol was registered with the Chinese Clinical Trial Registry (registration number: ChiCTR-COC-17013559). Plasma genomic DNA was extracted from all participants and analyzed for rs1801282 and rs3856806 single nucleotide polymorphisms using a SNaPshot Multiplex sequencing assay. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using unconditional logistic regression to estimate the association between ischemic stroke and a particular genotype. Results demonstrated that the G allele frequency of the PPARG gene rs1801282 locus was significantly higher in the case group than in the control group (P < 0.001). Individuals carrying the G allele had a 1.844 fold increased risk of ischemic stroke (OR = 1.844, 95% CI: 1.286-2.645, P < 0.001). Individuals carrying the rs3856806 T allele had a 1.366 fold increased risk of ischemic stroke (OR = 1.366, 95% CI: 1.077-1.733, P = 0.010). The distribution frequencies of the PPARG gene haplotypes rs1801282-rs3856806 in the control and case groups were determined. The frequency of distribution in the G-T haplotype case group was significantly higher than that in the control group. The risk of ischemic stroke increased to 2.953 times in individuals carrying the G-T haplotype (OR = 2.953, 95% CI: 2.082-4.190, P < 0.001). The rs1801282 G allele and rs3856806 T allele had a multiplicative interaction (OR = 3.404, 95% CI: 1.631-7.102, P < 0.001) and additive interaction (RERI = 41.705, 95% CI: 14.586-68.824, AP = 0.860; 95% CI: 0.779-0.940; S = 8.170, 95% CI: 3.772-17.697) on ischemic stroke risk, showing