Immune complex–driven neutrophil activation and BAFF release: a link to B cell responses in SLE

ObjectiveThe role of neutrophils in driving pathogenic B cell responses in SLE is not fully understood. In this study, we explored the link between immune complex (IC)–driven neutrophil activation, the release of B cell pro-survival factor BAFF and B cell activation using SLE clinical samples.Method...

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Published in:Lupus science & medicine 2022-07, Vol.9 (1), p.e000709
Main Authors: Wang, Ting, Vasconcellos, Andrew, Marken, John, Skopelja-Gardner, Sladjana, Lood, Christian, Giltiay, Natalia V
Format: Article
Language:English
Subjects:
Antigen-Antibody Complex
autoantibodies
B-Lymphocytes
Bioassays
Brief Communication
Cell culture
Cell growth
Cytokines
Experiments
Flow cytometry
Humans
Lupus Erythematosus, Systemic
Mann-Whitney U test
Neutrophil Activation
Neutrophils
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Summary:ObjectiveThe role of neutrophils in driving pathogenic B cell responses in SLE is not fully understood. In this study, we explored the link between immune complex (IC)–driven neutrophil activation, the release of B cell pro-survival factor BAFF and B cell activation using SLE clinical samples.MethodsBAFF levels were analysed in serum samples from patients with SLE (n=60) and healthy controls (HCs, n=20) by ELISA and correlated with markers of neutrophil activation and circulating IC levels. Neutrophils were stimulated with RNP/IgG ICs and neutrophil activation, the release of BAFF, and neutrophil-mediated B cell responses were studied in vitro.ResultsLevels of BAFF in patients with SLE were associated with markers of disease activity, including anti-dsDNA antibody titres (r=0.33, p
ISSN:2053-8790
2053-8790
DOI:10.1136/lupus-2022-000709