microRNA-193a-3p is specifically down-regulated and acts as a tumor suppressor in BRAF-mutated colorectal cancer

The aim of this study was to identify miRNAs specifically dysregulated in BRAF-mutated colorectal cancer, which could lead to a better understanding of the molecular mechanisms underlying oncogenesis of this malignant subtype of colorectal cancer. Candidate dysregulated miRNAs were selected in genom...

Full description

Saved in:
Bibliographic Details
Published in:BMC cancer 2017-11, Vol.17 (1), p.723-723, Article 723
Main Authors: Takahashi, Hidekazu, Takahashi, Masanobu, Ohnuma, Shinobu, Unno, Michiaki, Yoshino, Yuki, Ouchi, Kota, Takahashi, Shin, Yamada, Yasuhide, Shimodaira, Hideki, Ishioka, Chikashi
Format: Article
Language:English
Subjects:
Anti-EGFR therapy
BRAF
Colorectal cancer
Genetic aspects
Health aspects
Methods
MicroRNA
miR-193a-3p
miRNA
RNA sequencing
Tumor suppressor genes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study was to identify miRNAs specifically dysregulated in BRAF-mutated colorectal cancer, which could lead to a better understanding of the molecular mechanisms underlying oncogenesis of this malignant subtype of colorectal cancer. Candidate dysregulated miRNAs were selected in genome-wide miRNA expression array analysis using a screening set composed of 15 BRAF-mutated and 15 non-KRAS/BRAF-mutated colorectal cancers. The miRNA expressions were validated in another set of patients. The functional roles of the miRNAs were analyzed by cell growth and invasion assays. The association between miRNA expression status and the clinical outcome of patients treated with various chemotherapies was analyzed. Within the top five of the miRNAs screened, we validated miRNA-31 (miR-31) and miR-135b as up-regulated, while miR-193a-3p was down-regulated in BRAF-mutated cancer. Moreover, miR-193a-3p inhibited cell growth, and invasion of colorectal cancer cells. Low miR-193a-3p expression was associated with shorter progression-free survival in patients who received anti-EGFR therapy. Our results disclose a novel tumor suppressive role of miR-193a-3p in colorectal cancer. These results could lead to novel therapeutic strategies for colorectal cancer, particularly in BRAF-mutated colorectal cancer.
ISSN:1471-2407
1471-2407
DOI:10.1186/s12885-017-3739-x