Genetically Encoded Biosensors Based on Fluorescent Proteins

Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, differ...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2021-01, Vol.21 (3), p.795
Main Authors: Kim, Hyunbin, Ju, Jeongmin, Lee, Hae Nim, Chun, Hyeyeon, Seong, Jihye
Format: Article
Language:English
Subjects:
BiFC
Biosensing Techniques
Biosensors
ddFP
Design
Energy transfer
Fluorescence
Fluorescence Resonance Energy Transfer
Fluorescent Dyes
fluorescent protein
FRET
Genetic code
genetically encoded biosensor
Luminescent Proteins - genetics
Molecular dynamics
Plasma
Proteins
Review
Sensors
split FP
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Summary:Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, different sensing strategies need to be applied for the best design of FP-based biosensors. Here, we review genetically encoded biosensors based on FPs with various sensing strategies, for example, translocation, fluorescence resonance energy transfer (FRET), reconstitution of split FP, pH sensitivity, maturation speed, and so on. We introduce general principles of each sensing strategy and discuss critical factors to be considered if available, then provide representative examples of these FP-based biosensors. These will help in designing the best sensing strategy for the successful development of new genetically encoded biosensors based on FPs.
ISSN:1424-8220
1424-8220
DOI:10.3390/s21030795