Translocation of Drought-Responsive Proteins from the Chloroplasts

• Published in: Cells (Basel, Switzerland) Switzerland), 2020-01, Vol.9 (1), p.259
• Main Authors: Li, Ping, Liu, Haoju, Yang, Hong, Pu, Xiaojun, Li, Chuanhong, Huo, Heqiang, Chu, Zhaohui, Chang, Yuxiao, Lin, Yongjun, Liu, Li
• Format: Article
• Language: English
• Subjects: Agricultural production; Arabidopsis thaliana; chloroplast; Chloroplast Proteins - metabolism; Chloroplasts; Chloroplasts - metabolism; Communication; Cytosol; Drought; Droughts; Environmental conditions; Gene expression; Investigations; itraq; Metabolism; Metabolites; Nicotiana - metabolism; Nicotiana - physiology; Nicotiana benthamiana; Oryza sativa; Osmotic stress; Physcomitrella patens; Plant growth; Plant Leaves - metabolism; Protein Transport; Proteins; proteomics; Protoplasts; Quantitation; retrograde signal; Retrograde transport; Signal Transduction; Stress, Physiological; Tobacco; Water
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells9010259

Some chloroplast proteins are known to serve as messengers to transmit retrograde signals from chloroplasts to the nuclei in response to environmental stresses. However, whether particular chloroplast proteins respond to drought stress and serve as messengers for retrograde signal transduction are unclear. Here, we used isobaric tags for relative and absolute quantitation (iTRAQ) to monitor the proteomic changes in tobacco ( ) treated with drought stress/re-watering. We identified 3936 and 1087 differentially accumulated total leaf and chloroplast proteins, respectively, which were grouped into 16 categories. Among these, one particular category of proteins, that includes carbonic anhydrase 1 (CA1), exhibited a great decline in chloroplasts, but a remarkable increase in leaves under drought stress. The subcellular localizations of CA1 proteins from moss ( ), and rice ( ) in protoplasts consistently showed that CA1 proteins gradually diminished within chloroplasts but increasingly accumulated in the cytosol under osmotic stress treatment, suggesting that they could be translocated from chloroplasts to the cytosol and act as a signal messenger from the chloroplast. Our results thus highlight the potential importance of chloroplast proteins in retrograde signaling pathways and provide a set of candidate proteins for further research.