In vivo Analysis of H2AX Phosphorylation Induced by γ-Radiation

ABSTRACT          Background and Objectives: Exposure to ionizing radiation in modern societies is inevitable and can cause a variety of adverse health effects such as cancer and birth defects. Therefore, a reliable, repeatable and sensitive method is required for evaluation of radiation exposure. T...

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Published in:Medical laboratory journal 2017-03, Vol.11 (2), p.11-15
Main Authors: Seyed Mostafa Mir, Esmaeil Samadian, Seyed Hossein Sadeghi, Alireza Khoshbin khoshnazar
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Language:English
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ionizing radiation
mouse
γh2ax protein
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container_title Medical laboratory journal
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creator Seyed Mostafa Mir
Esmaeil Samadian
Seyed Hossein Sadeghi
Alireza Khoshbin khoshnazar
description ABSTRACT          Background and Objectives: Exposure to ionizing radiation in modern societies is inevitable and can cause a variety of adverse health effects such as cancer and birth defects. Therefore, a reliable, repeatable and sensitive method is required for evaluation of radiation exposure. The aim of this study was to determine the amount of histone H2AX phosphorylation as an indicator of radiation exposure to evaluate the rate of double-strand DNA breakage in irradiated mice.          Methods: In this study, 15 mice were exposed to different doses of ionizing radiation. After extraction of total protein from bone marrow cells, γH2AX protein was measured by western blotting. Data analysis was performed using ANOVA, Tukeychr('39')s post hoc test, and the Pearsonchr('39')s correlation test.          Results: The amount of γH2AX protein in the exposed groups increased significantly compared to the control group (P
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Therefore, a reliable, repeatable and sensitive method is required for evaluation of radiation exposure. The aim of this study was to determine the amount of histone H2AX phosphorylation as an indicator of radiation exposure to evaluate the rate of double-strand DNA breakage in irradiated mice.          Methods: In this study, 15 mice were exposed to different doses of ionizing radiation. After extraction of total protein from bone marrow cells, γH2AX protein was measured by western blotting. Data analysis was performed using ANOVA, Tukeychr('39')s post hoc test, and the Pearsonchr('39')s correlation test.          Results: The amount of γH2AX protein in the exposed groups increased significantly compared to the control group (P&lt;0.05).         Conclusion: The results of this study indicate that exposure to ionizing radiation increases the amount of γH2AX protein in bone marrow cells during the early hours. 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subjects ionizing radiation
mouse
γh2ax protein
title In vivo Analysis of H2AX Phosphorylation Induced by γ-Radiation
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