Evolution, heterogeneity and global dispersal of cosmopolitan genotype of Dengue virus type 2

• Published in: Scientific reports 2021-06, Vol.11 (1), p.13496-15, Article 13496
• Main Authors: Yenamandra, Surya Pavan, Koo, Carmen, Chiang, Suzanna, Lim, Han Shi Jeri, Yeo, Zhen Yuan, Ng, Lee Ching, Hapuarachchi, Hapuarachchige Chanditha
• Format: Article
• Language: English
• Subjects: 631/181/735; 631/181/757; 631/326/596/1413; Amino acids; Dengue - epidemiology; Dengue - genetics; Dengue fever; Dengue virus; Dengue Virus - genetics; Dispersal; Divergence; Env gene; Epidemiology; Evolution; Evolution, Molecular; Genomic analysis; Genotype; Genotype & phenotype; Genotypes; Heterogeneity; Humanities and Social Sciences; Humans; multidisciplinary; Phylogeny; Science; Science (multidisciplinary); Spatial analysis; Tropical environments; Vector-borne diseases; Viral envelope proteins; Viral Envelope Proteins - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-92783-y

Dengue virus type 2 (DENV-2) contributes substantially to the dengue burden and dengue-related mortality in the tropics and sub-tropics. DENV-2 includes six genotypes, among which cosmopolitan genotype is the most widespread. The present study investigated the evolution, intra-genotype heterogeneity and dispersal of cosmopolitan genotype to understand unique genetic characteristics that have shaped the molecular epidemiology and distribution of cosmopolitan lineages. The spatial analysis demonstrated a wide geo-distribution of cosmopolitan genotype through an extensive inter-continental network, anchored in Southeast Asia and Indian sub-continent. Intra-genotype analyses using 3367 envelope gene sequences revealed six distinct lineages within the cosmopolitan genotype, namely the Indian sub-continent lineage and five other lineages. Indian sub-continent lineage was the most diverged among six lineages and has almost reached the nucleotide divergence threshold of 6% within E gene to qualify as a separate genotype. Genome wide amino acid signatures and selection pressure analyses further suggested differences in evolutionary characteristics between the Indian sub-continent lineage and other lineages. The present study narrates a comprehensive genomic analysis of cosmopolitan genotype and presents notable genetic characteristics that occurred during its evolution and global expansion. Whether those characteristics conferred a fitness advantage to cosmopolitan genotype in different geographies warrant further investigations.