Enhanced anti-inflammatory effects and metabolic profiles of cyanidin-3-glucoside via nanoliposomal encapsulation in Caco-2 and RAW 264.7 co-culture system

Cyanidin-3-glucoside (C3G) has been found to prevent/treat intestinal disease and maintain gut homeostasis. Numerous studies have proved that nanoliposomes can improve the absorption and utilization of food nutrients. However, the impacts of C3G nanoliposomes (C3G-NL) are still unkown. This study in...

Full description

Saved in:
Bibliographic Details
Published in:Journal of functional foods 2024-12, Vol.123, p.106588, Article 106588
Main Authors: Zhong, Hao, Luo, Xin, Yang, Mengyu, Zhao, Bo, Liu, Xiaofeng, Hussain, Muhammad, Hu, Chaofan, Abdullah, Guan, Rongfa
Format: Article
Language:English
Subjects:
Anti-inflammatory activity
Co-culture model
Cyanidin-3-glucoside
Nanoliposomes
Targeted metabolomics
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cyanidin-3-glucoside (C3G) has been found to prevent/treat intestinal disease and maintain gut homeostasis. Numerous studies have proved that nanoliposomes can improve the absorption and utilization of food nutrients. However, the impacts of C3G nanoliposomes (C3G-NL) are still unkown. This study investigated the effects of cyanidin-3-glucoside nanoliposomes (C3G-NL) on intestinal inflammation and permeability using a Caco-2/RAW264.7 co-culture model. ELISA and qPCR results showed that C3G-NL reduced pro-inflammatory markers, including TNF-α, IL-6, IL-8, cyclooxygenase-2, and inducible nitric oxide synthase, while inhibiting the NF-κB/MAPK pathway. C3G-NL also improved intestinal barrier integrity by increasing transepithelial electrical resistance and upregulating tight junction proteins (zonula occludens-1, occludin, claudin-1). Additionally, C3G-NL enhanced C3G metabolism, leading to the formation of intermediary metabolites like chalcone and dihydroflavonols, which contributed to the synthesis of other anthocyanins (delphinidin, peonidin, petunidin, malvidin, and pelargonidin). Overall, C3G-NL helps mitigate inflammation and restore tight junction integrity via metabolic regulation. [Display omitted] •A short-term co-culture intestinal inflammatory cell model is established.•C3G-NL down-regulate the expression of inflammatory markers and NF-κB/MAPK pathway.•C3G-NL could protect intestinal health by increasing tight junction proteins.•Metabolomics analysis reveals that C3G-NL could be effectively metabolized.
ISSN:1756-4646
DOI:10.1016/j.jff.2024.106588