Cyclodextrin Nanosponges Inclusion Compounds Associated with Gold Nanoparticles for Potential Application in the Photothermal Release of Melphalan and Cytoxan

• Published in: International journal of molecular sciences 2021-06, Vol.22 (12), p.6446
• Main Authors: Salazar, Sebastián, Yutronic, Nicolás, Kogan, Marcelo J, Jara, Paul
• Format: Article
• Language: English
• Subjects: anti-tumor; Cancer therapies; Cavities; Chemistry Techniques, Synthetic; Crosslinking; cyclodextrin polymers; Cyclodextrins; Cyclodextrins - chemistry; Cyclophosphamide - administration & dosage; Drug Carriers - chemistry; Drug delivery; Drug Delivery Systems; Drug Liberation - radiation effects; Drugs; Gold; Gold - chemistry; Irradiation; Light; Magnetic Resonance Spectroscopy; Melphalan; Melphalan - administration & dosage; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Morphology; Nanoparticles; NMR; Nuclear magnetic resonance; photothermal therapy; plasmon surface resonance; Scanning electron microscopy; Spectroscopy; Spectrum analysis; Temperature; Thermogravimetric analysis; Thermogravimetry; Tocopherols; X ray powder diffraction; X-Ray Diffraction; β-Cyclodextrin
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22126446

This article describes the synthesis and characterization of β-cyclodextrin-based nano-sponges (NS) inclusion compounds (IC) with the anti-tumor drugs melphalan (MPH) and cytoxan (CYT), and the addition of gold nanoparticles (AuNPs) onto both systems, for the potential release of the drugs by means of laser irradiation. The NS-MPH and NS-CYT inclusion compounds were characterized using scanning electron microscopy (SEM), X-ray powder diffraction (XRPD), energy dispersive spectroscopy (EDS), thermogravimetric analysis (TGA), UV-Vis, and proton nuclear magnetic resonance ( H-NMR). Thus, the inclusion of MPH and CYT inside the cavities of NSs was confirmed. The association of AuNPs with the ICs was confirmed by SEM, EDS, TEM, and UV-Vis. Drug release studies using NSs synthesized with different molar ratios of β-cyclodextrin and diphenylcarbonate (1:4 and 1:8) demonstrated that the ability of NSs to entrap and release the drug molecules depends on the crosslinking between the cyclodextrin monomers. Finally, irradiation assays using a continuous laser of 532 nm showed that photothermal drug release of both MPH and CYT from the cavities of NSs via plasmonic heating of AuNPs is possible.