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The effect of CA1 administration of orexin-A on hippocampal expression of COX-2 and BDNF in a rat model of orofacial pain
ABSTRACT The neuropeptide orexin-A and its receptors are widely distributed in both hippocampal circuitry and pain transmission pathways. Objective: Involvement of the CA1 orexin 1 receptor (OX1R) on the modulation of orofacial pain and pain-induced changes in hippocampal expression of cyclooxygenas...
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| Published in: | Arquivos de neuro-psiquiatria 2018-09, Vol.76 (9), p.603-608 |
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| container_title | Arquivos de neuro-psiquiatria |
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| creator | Kooshki, Razieh Abbasnejad, Mehdi Esmaeili-Mahani, Saeed Raoof, Maryam |
| description | ABSTRACT
The neuropeptide orexin-A and its receptors are widely distributed in both hippocampal circuitry and pain transmission pathways.
Objective:
Involvement of the CA1 orexin 1 receptor (OX1R) on the modulation of orofacial pain and pain-induced changes in hippocampal expression of cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) was investigated.
Methods:
Orofacial pain was induced by an intra-lip injection of capsaicin (100 μg). Reverse transcription polymerase chain reaction and immunoblot analysis were used to indicate changes in hippocampal BDNF and COX-2 expression, respectively.
Results:
Capsaicin induces a significant pain response, which is not affected by either orexin-A or SB-334867-A, an OX1R antagonist. However, an increased expression of COX-2 and decreased expression of BDNF was observed in the hippocampus of animals that received capsaicin or SB-334867-A (80 nM) plus capsaicin. Meanwhile, orexin-A (40 pM) attenuated the effects of capsaicin on the expression of COX-2 and BDNF.
Conclusions:
CA1 OX1R activation moderates capsaicin-induced neuronal inflammation and neurotrophic deficiency. |
| doi_str_mv | 10.1590/0004-282X20180099 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_47c0d851d9274733b1838ef3cda09806</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><scielo_id>S0004_282X2018000900603</scielo_id><doaj_id>oai_doaj_org_article_47c0d851d9274733b1838ef3cda09806</doaj_id><sourcerecordid>2161056090</sourcerecordid><originalsourceid>FETCH-LOGICAL-c544t-b3310cef4ad6b50545286f6a1befc15f2a99f7dbfcc56e88df8e7ffb0b96db0b3</originalsourceid><addsrcrecordid>eNpdkk-P1CAYxhujccfVD-DFkHjx0vUFWgrHcXR1k417cE32Rih_HCZtqdBJZr-9jJ3pJnuB8PI8P17gKYr3GK5wLeAzAFQl4eSBAOYAQrwoVpg1vKwIaV4Wq2X_oniT0g6AVEI0r4sLCpTVjFSr4vF-a5F1zuoJBYc2a4yU6f3g0xTV5MNwrIZoD34o1ygvt34cg1b9qDpkD2O0KZ1Um7uHkiA1GPTl689r5AekUGagPhjbzZjglPbZOCo_vC1eOdUl--40Xxa_r7_db36Ut3ffbzbr21LXVTWVLaUYtHWVMqytoa5qwpljCrfWaVw7ooRwjWmd1jWznBvHbeNcC61gJo_0sriZuSaonRyj71V8lEF5-b8Q4h-p4uR1Z2XVaDC8xkaQpmoobTGn3DqqjQLBgWXW1cxK2tsuyF3YxyE3L38dn1o-fQUIAAY0Gz7NhjGGv3ubJtn7pG3XqcGGfZIEE5alFRZZ-vGZdKETzDDULEOzCs8qHUNK0brlRhjkMRTy3MnhHIrs-XAi79vemsVxTkEWwCyYtt729ung58wlaPQfxXK7rg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2161056090</pqid></control><display><type>article</type><title>The effect of CA1 administration of orexin-A on hippocampal expression of COX-2 and BDNF in a rat model of orofacial pain</title><source>SciELO Brazil</source><creator>Kooshki, Razieh ; Abbasnejad, Mehdi ; Esmaeili-Mahani, Saeed ; Raoof, Maryam</creator><creatorcontrib>Kooshki, Razieh ; Abbasnejad, Mehdi ; Esmaeili-Mahani, Saeed ; Raoof, Maryam</creatorcontrib><description>ABSTRACT
The neuropeptide orexin-A and its receptors are widely distributed in both hippocampal circuitry and pain transmission pathways.
Objective:
Involvement of the CA1 orexin 1 receptor (OX1R) on the modulation of orofacial pain and pain-induced changes in hippocampal expression of cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) was investigated.
Methods:
Orofacial pain was induced by an intra-lip injection of capsaicin (100 μg). Reverse transcription polymerase chain reaction and immunoblot analysis were used to indicate changes in hippocampal BDNF and COX-2 expression, respectively.
Results:
Capsaicin induces a significant pain response, which is not affected by either orexin-A or SB-334867-A, an OX1R antagonist. However, an increased expression of COX-2 and decreased expression of BDNF was observed in the hippocampus of animals that received capsaicin or SB-334867-A (80 nM) plus capsaicin. Meanwhile, orexin-A (40 pM) attenuated the effects of capsaicin on the expression of COX-2 and BDNF.
Conclusions:
CA1 OX1R activation moderates capsaicin-induced neuronal inflammation and neurotrophic deficiency.</description><identifier>ISSN: 0004-282X</identifier><identifier>ISSN: 1678-4227</identifier><identifier>EISSN: 1678-4227</identifier><identifier>DOI: 10.1590/0004-282X20180099</identifier><identifier>PMID: 30365624</identifier><language>eng</language><publisher>Rua do Matoso 170, Rio de Janeiro, RJ, CEP 20270-135, Brazil: Thieme Revinter Publicações Ltda</publisher><subject>Animals ; Benzoxazoles - pharmacology ; Brain-derived neurotrophic factor ; Brain-Derived Neurotrophic Factor - metabolism ; Capsaicin ; Cyclooxygenase 2 - metabolism ; Cyclooxygenase-2 ; Disease Models, Animal ; Facial Pain - metabolism ; Hippocampus ; Hippocampus - drug effects ; Hippocampus - metabolism ; Male ; Neurons - drug effects ; Neurons - metabolism ; NEUROSCIENCES ; Orexin Receptors - metabolism ; Orexins ; Orexins - pharmacology ; Orofacial pain ; Pain ; Polymerase chain reaction ; PSYCHIATRY ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; Reverse transcription ; Urea - analogs & derivatives ; Urea - pharmacology</subject><ispartof>Arquivos de neuro-psiquiatria, 2018-09, Vol.76 (9), p.603-608</ispartof><rights>Academia Brasileira de Neurologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon.</rights><rights>Copyright Arquivos de Neuro-Psiquiatria Sep 2018</rights><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-b3310cef4ad6b50545286f6a1befc15f2a99f7dbfcc56e88df8e7ffb0b96db0b3</citedby><cites>FETCH-LOGICAL-c544t-b3310cef4ad6b50545286f6a1befc15f2a99f7dbfcc56e88df8e7ffb0b96db0b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,24150,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30365624$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kooshki, Razieh</creatorcontrib><creatorcontrib>Abbasnejad, Mehdi</creatorcontrib><creatorcontrib>Esmaeili-Mahani, Saeed</creatorcontrib><creatorcontrib>Raoof, Maryam</creatorcontrib><title>The effect of CA1 administration of orexin-A on hippocampal expression of COX-2 and BDNF in a rat model of orofacial pain</title><title>Arquivos de neuro-psiquiatria</title><addtitle>Arq Neuropsiquiatr</addtitle><description>ABSTRACT
The neuropeptide orexin-A and its receptors are widely distributed in both hippocampal circuitry and pain transmission pathways.
Objective:
Involvement of the CA1 orexin 1 receptor (OX1R) on the modulation of orofacial pain and pain-induced changes in hippocampal expression of cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) was investigated.
Methods:
Orofacial pain was induced by an intra-lip injection of capsaicin (100 μg). Reverse transcription polymerase chain reaction and immunoblot analysis were used to indicate changes in hippocampal BDNF and COX-2 expression, respectively.
Results:
Capsaicin induces a significant pain response, which is not affected by either orexin-A or SB-334867-A, an OX1R antagonist. However, an increased expression of COX-2 and decreased expression of BDNF was observed in the hippocampus of animals that received capsaicin or SB-334867-A (80 nM) plus capsaicin. Meanwhile, orexin-A (40 pM) attenuated the effects of capsaicin on the expression of COX-2 and BDNF.
Conclusions:
CA1 OX1R activation moderates capsaicin-induced neuronal inflammation and neurotrophic deficiency.</description><subject>Animals</subject><subject>Benzoxazoles - pharmacology</subject><subject>Brain-derived neurotrophic factor</subject><subject>Brain-Derived Neurotrophic Factor - metabolism</subject><subject>Capsaicin</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Cyclooxygenase-2</subject><subject>Disease Models, Animal</subject><subject>Facial Pain - metabolism</subject><subject>Hippocampus</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - metabolism</subject><subject>Male</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>NEUROSCIENCES</subject><subject>Orexin Receptors - metabolism</subject><subject>Orexins</subject><subject>Orexins - pharmacology</subject><subject>Orofacial pain</subject><subject>Pain</subject><subject>Polymerase chain reaction</subject><subject>PSYCHIATRY</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Reverse transcription</subject><subject>Urea - analogs & derivatives</subject><subject>Urea - pharmacology</subject><issn>0004-282X</issn><issn>1678-4227</issn><issn>1678-4227</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>0U6</sourceid><sourceid>DOA</sourceid><recordid>eNpdkk-P1CAYxhujccfVD-DFkHjx0vUFWgrHcXR1k417cE32Rih_HCZtqdBJZr-9jJ3pJnuB8PI8P17gKYr3GK5wLeAzAFQl4eSBAOYAQrwoVpg1vKwIaV4Wq2X_oniT0g6AVEI0r4sLCpTVjFSr4vF-a5F1zuoJBYc2a4yU6f3g0xTV5MNwrIZoD34o1ygvt34cg1b9qDpkD2O0KZ1Um7uHkiA1GPTl689r5AekUGagPhjbzZjglPbZOCo_vC1eOdUl--40Xxa_r7_db36Ut3ffbzbr21LXVTWVLaUYtHWVMqytoa5qwpljCrfWaVw7ooRwjWmd1jWznBvHbeNcC61gJo_0sriZuSaonRyj71V8lEF5-b8Q4h-p4uR1Z2XVaDC8xkaQpmoobTGn3DqqjQLBgWXW1cxK2tsuyF3YxyE3L38dn1o-fQUIAAY0Gz7NhjGGv3ubJtn7pG3XqcGGfZIEE5alFRZZ-vGZdKETzDDULEOzCs8qHUNK0brlRhjkMRTy3MnhHIrs-XAi79vemsVxTkEWwCyYtt729ung58wlaPQfxXK7rg</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Kooshki, Razieh</creator><creator>Abbasnejad, Mehdi</creator><creator>Esmaeili-Mahani, Saeed</creator><creator>Raoof, Maryam</creator><general>Thieme Revinter Publicações Ltda</general><general>Arquivos de Neuro-Psiquiatria</general><general>Academia Brasileira de Neurologia - ABNEURO</general><general>Academia Brasileira de Neurologia (ABNEURO)</general><scope>0U6</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>20180901</creationdate><title>The effect of CA1 administration of orexin-A on hippocampal expression of COX-2 and BDNF in a rat model of orofacial pain</title><author>Kooshki, Razieh ; Abbasnejad, Mehdi ; Esmaeili-Mahani, Saeed ; Raoof, Maryam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c544t-b3310cef4ad6b50545286f6a1befc15f2a99f7dbfcc56e88df8e7ffb0b96db0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Benzoxazoles - pharmacology</topic><topic>Brain-derived neurotrophic factor</topic><topic>Brain-Derived Neurotrophic Factor - metabolism</topic><topic>Capsaicin</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Cyclooxygenase-2</topic><topic>Disease Models, Animal</topic><topic>Facial Pain - metabolism</topic><topic>Hippocampus</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - metabolism</topic><topic>Male</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>NEUROSCIENCES</topic><topic>Orexin Receptors - metabolism</topic><topic>Orexins</topic><topic>Orexins - pharmacology</topic><topic>Orofacial pain</topic><topic>Pain</topic><topic>Polymerase chain reaction</topic><topic>PSYCHIATRY</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Reverse transcription</topic><topic>Urea - analogs & derivatives</topic><topic>Urea - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kooshki, Razieh</creatorcontrib><creatorcontrib>Abbasnejad, Mehdi</creatorcontrib><creatorcontrib>Esmaeili-Mahani, Saeed</creatorcontrib><creatorcontrib>Raoof, Maryam</creatorcontrib><collection>Thieme_OA刊</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>SciELO</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Arquivos de neuro-psiquiatria</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kooshki, Razieh</au><au>Abbasnejad, Mehdi</au><au>Esmaeili-Mahani, Saeed</au><au>Raoof, Maryam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of CA1 administration of orexin-A on hippocampal expression of COX-2 and BDNF in a rat model of orofacial pain</atitle><jtitle>Arquivos de neuro-psiquiatria</jtitle><addtitle>Arq Neuropsiquiatr</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>76</volume><issue>9</issue><spage>603</spage><epage>608</epage><pages>603-608</pages><issn>0004-282X</issn><issn>1678-4227</issn><eissn>1678-4227</eissn><abstract>ABSTRACT
The neuropeptide orexin-A and its receptors are widely distributed in both hippocampal circuitry and pain transmission pathways.
Objective:
Involvement of the CA1 orexin 1 receptor (OX1R) on the modulation of orofacial pain and pain-induced changes in hippocampal expression of cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) was investigated.
Methods:
Orofacial pain was induced by an intra-lip injection of capsaicin (100 μg). Reverse transcription polymerase chain reaction and immunoblot analysis were used to indicate changes in hippocampal BDNF and COX-2 expression, respectively.
Results:
Capsaicin induces a significant pain response, which is not affected by either orexin-A or SB-334867-A, an OX1R antagonist. However, an increased expression of COX-2 and decreased expression of BDNF was observed in the hippocampus of animals that received capsaicin or SB-334867-A (80 nM) plus capsaicin. Meanwhile, orexin-A (40 pM) attenuated the effects of capsaicin on the expression of COX-2 and BDNF.
Conclusions:
CA1 OX1R activation moderates capsaicin-induced neuronal inflammation and neurotrophic deficiency.</abstract><cop>Rua do Matoso 170, Rio de Janeiro, RJ, CEP 20270-135, Brazil</cop><pub>Thieme Revinter Publicações Ltda</pub><pmid>30365624</pmid><doi>10.1590/0004-282X20180099</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0004-282X 1678-4227 1678-4227 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_47c0d851d9274733b1838ef3cda09806 |
| source | SciELO Brazil |
| subjects | Animals Benzoxazoles - pharmacology Brain-derived neurotrophic factor Brain-Derived Neurotrophic Factor - metabolism Capsaicin Cyclooxygenase 2 - metabolism Cyclooxygenase-2 Disease Models, Animal Facial Pain - metabolism Hippocampus Hippocampus - drug effects Hippocampus - metabolism Male Neurons - drug effects Neurons - metabolism NEUROSCIENCES Orexin Receptors - metabolism Orexins Orexins - pharmacology Orofacial pain Pain Polymerase chain reaction PSYCHIATRY Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Reverse transcription Urea - analogs & derivatives Urea - pharmacology |
| title | The effect of CA1 administration of orexin-A on hippocampal expression of COX-2 and BDNF in a rat model of orofacial pain |
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