CLPTM1L Is a Novel Putative Oncogene Promoting Tumorigenesis in Oral Squamous Cell Carcinoma

This study aimed to explore the function of CLPTM1L in oral squamous cell carcinoma and mechanism of tumorigenesis. The expression of CLPTM1L was detected by immunohistochemistry. The localization in cells was detected by immunofluorescence. Cell invasion, proliferation, and migration were detected...

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Bibliographic Details
Published in:Cell transplantation 2021, Vol.30, p.9636897211045970-9636897211045970
Main Authors: Hou, Yunwen, Xue, Feifei, Fu, Yu, Feng, Guanying, Wang, Ruixia, Yuan, Hua
Format: Article
Language:English
Subjects:
Animals
Cancer
Carcinogenesis
Carcinoma, Squamous Cell - genetics
Cell Line, Tumor
Cell migration
Cell Proliferation
Cholecystokinin
Cohort Studies
Cytoplasm
Endoplasmic reticulum
Epithelium
Female
Humans
Hydroxylation
Immunofluorescence
Immunohistochemistry
Localization
Membrane Proteins - metabolism
Mice
Middle Aged
Mouth Neoplasms - genetics
Oncogenes
Oncogenes - genetics
Oral cancer
Oral carcinoma
Oral squamous cell carcinoma
Original
Prognosis
Protein biosynthesis
Protein folding
Proteins
Retrospective Studies
Risk factors
Squamous cell carcinoma
Transfection
Tumorigenesis
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Summary:This study aimed to explore the function of CLPTM1L in oral squamous cell carcinoma and mechanism of tumorigenesis. The expression of CLPTM1L was detected by immunohistochemistry. The localization in cells was detected by immunofluorescence. Cell invasion, proliferation, and migration were detected by transwell, CCK-8 and scratch-wound test. The possible characteristics of CLPTM1L were analysed in TCGA, GO, KEGG and String databases. IHC revealed that the expression of CLPTM1L in 92 cases of OSCC tissues was significantly higher (P < 0.01) than 29 cases of normal oral epithelium tissues. The expression of CLPTM1L was significantly higher in oral squamous cell carcinoma in TCGA database. CLPTM1L expression was not significantly correlated with the patients’ clinical parameters. High expression of CLPTM1L was associated with worse prognosis. Cox regression analysis demonstrated that the CLPTM1L expression was the significant risk factor. CLPTM1L was mainly localized in the perinuclear cytoplasm. The vitro studies revealed that the knockdown of CLPTM1L suppressed invasion, proliferation and migration. CLPTM1L related genes were enriched in protein processing in the endoplasmic reticulum, protein folding, endoplasmic reticulum formation, N-glycan biosynthesis, and protein hydroxylation. Highly expressed CLPTM1L may contribute to a poor prognosis and increase invasion, proliferation and migration of oral squamous cell carcinoma. CLPTM1L may play an important role in tumorgenesis and would be a valuable target gene for the treatment of oral squamous cell carcinoma.
ISSN:0963-6897
1555-3892
DOI:10.1177/09636897211045970