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pH-Responsive Collagen Hydrogels Prepared by UV Irradiation in the Presence of Riboflavin

This study reveals the pH-responsive behavior of collagen hydrogels prepared using ultraviolet (UV) irradiation with riboflavin as a photosensitizer. By varying the UV exposure time, we modulated the crosslinking density, thereby influencing the mechanical properties and pH responsiveness. Rheologic...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-09, Vol.25 (19), p.10439
Main Authors: Setoyama, Shoki, Haraguchi, Ryota, Aoki, Shigehisa, Oishi, Yushi, Narita, Takayuki
Format: Article
Language:English
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Summary:This study reveals the pH-responsive behavior of collagen hydrogels prepared using ultraviolet (UV) irradiation with riboflavin as a photosensitizer. By varying the UV exposure time, we modulated the crosslinking density, thereby influencing the mechanical properties and pH responsiveness. Rheological analysis confirmed successful network formation, whereas swelling studies revealed significant pH-dependent behavior, with maximum swelling at a pH of four and minimal swelling above a pH of six, demonstrating partial reversibility over multiple pH cycles. Mechanical testing showed a pH-dependent elastic modulus, which increased 10 fold from a pH of 6 to 10. Fibroblast proliferation assays confirmed the biocompatibility of the hydrogels, with cell growth positively correlating with the UV exposure time. This research demonstrates the potential of UV-crosslinked collagen hydrogels in biomedical applications, such as tissue engineering and drug delivery, where pH responsiveness is essential.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms251910439