Development of a fluorescent quantification strip assay for the detection of lead

Lead (Pb) is a toxic metal that can accumulate in the body and negatively impact human health. In this study, we prepared a highly sensitive and specific monoclonal antibody, 2C3, that can recognize Pb(II) with an IC50 and linear range of 0.5 ng/mL and 0.08-1.86 ng/mL, respectively. Additionally, ba...

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Bibliographic Details
Published in:Food and agricultural immunology 2020-01, Vol.31 (1), p.642-652
Main Authors: Li, Shaozhen, Wu, Xiaoling, Kuang, Hua, Zhu, Jianping, Liu, Liqiang
Format: Article
Language:English
Subjects:
Fluorescence
fluorescent quantification
Lead
Monoclonal antibodies
monoclonal antibody
Strip
test strip
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Summary:Lead (Pb) is a toxic metal that can accumulate in the body and negatively impact human health. In this study, we prepared a highly sensitive and specific monoclonal antibody, 2C3, that can recognize Pb(II) with an IC50 and linear range of 0.5 ng/mL and 0.08-1.86 ng/mL, respectively. Additionally, based on this antibody we developed a fluorescent test strip, which can be used for both qualitative and quantitative detection of Pb(II) in brown rice with a detection range of 4.67-47.8 ng/mL. Therefore, the method we developed has high sensitivity suitable for the detection of Pb(II) in brown rice.
ISSN:0954-0105
1465-3443
DOI:10.1080/09540105.2020.1740179