Enzymatically Hydrolysed Molasses and Sodium Citrate as New Potentials for the Improvement of Canthaxanthin Batch Synthesis by Dietzia natronolimnaea HS-1: A statistical Media Optimisation

• Published in: Czech Journal of Food Sciences 2014-01, Vol.32 (4), p.326-336
• Main Authors: Gharibzahedi, S.M.T., University of Tehran (Iran). Dept. of Food Science, Engineering and Technology, Razavi, S.H., University of Tehran (Iran). Dept. of Food Science, Engineering and Technology, Mousavi, M., University of Tehran (Iran). Dept. of Food Science, Engineering and Technology
• Format: Article
• Language: English
• Subjects: ABSORBANCE; ABSORBANCIA; ANALYTICAL METHODS; BACTERIA GRAM NEGATIVA; BACTERIE GRAM NEGATIF; batch submerged fermentation; BIOLOGICAL PRODUCTION; BIOMASA; BIOMASS; BIOMASSE; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; carotenoid pigment; CAROTENOIDE; CAROTENOIDES; CAROTENOIDS; Citric acid; COLORANT; COLORANTES; CRECIMIENTO; CROISSANCE; CULTURE MEDIA; Dietzia; DISENO EXPERIMENTAL; DISPOSITIF EXPERIMENTAL; DYES; ENZYMATIC HYDROLYSIS; ESPECTROMETRIA; EXPERIMENTAL DESIGN; EXPERIMENTAL INFECTION; EXTRACCION; EXTRACTION; GRAM NEGATIVE BACTERIA; GROWTH; HIDROLISIS ENZIMATICA; HYDROLYSE ENZYMATIQUE; INFECCION EXPERIMENTAL; INFECTION EXPERIMENTALE; MATHEMATICAL MODELS; MEDIO DE CULTIVO; MELASSE; MELAZA; MILIEU DE CULTURE; MODELE MATHEMATIQUE; MODELOS MATEMATICOS; MOLASSES; NATURAL RESOURCES; Optimization; PESO; PIGMENT; PIGMENTOS; PIGMENTS; POIDS; Polynomials; PRODUCCION BIOLOGICA; PRODUCTION BIOLOGIQUE; RECURSOS NATURALES; Response surface methodology; response surface modelling; RESSOURCE NATURELLE; Sodium; Sodium citrate; SOLUTE; SOLUTES; SOLUTO; SOUS-PRODUIT DE SUCRERIE; SPECTROMETRIE; SPECTROMETRY; Statistical analysis; SUBPRODUCTOS DEL AZUCAR; SUGAR BYPRODUCTS; sugarbeet molasses hydrolysate; Syrups & sweeteners; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; WEIGHT; Yeast; Yeasts
• ISSN: 1212-1800; 1805-9317
• DOI: 10.17221/472/2013-cjfs

Response surface methodology-central composite rotatable design (RSM-CCRD) was applied to explore the optimum media formulation for maximising canthaxanthin (CTX) biosynthesis by Dietzia natronolimnaea HS-1. The effects of three variables of enzymatically hydrolysed molasses (EHM) (16.6-33.4 g/l), sodium (Na)-citrate (21.64-28.36mM), and yeast extract (6.32-9.68 g/l) concentrations on the production of CTX, total carotenoid (TCT), and biomass dry weight (BDW) were appraised. The results showed that the quadratic effects of EHM, yeast extract, and Na-citrate contents in terms of second-order polynomial regression equations (R2 = 0.968-0.986), respectively, had the most significant effects on the produced TCT and CTX. The predicted maximum accumulation of BDW (8.88 g/l), TCT (7.24 mg/l), and CTX (6.40 mg/l) under the optimum concentrations of the media variables (26.16 g/l EHM, 8.29 g/l yeast extract, and 25.86mM Na-citrate) was very close to the experimental values determined in batch experiments. The high BDW content suggested EHM and Na-citrate as very promising feedstocks for CTX bioproduction by the bacterium studied.