Comprehensive microRNA-seq transcriptomic profiling across 11 organs, 4 ages, and 2 sexes of Fischer 344 rats

Rat is one of the most widely-used models in chemical safety evaluation and biomedical research. However, the knowledge about its microRNA (miRNA) expression patterns across multiple organs and various developmental stages is still limited. Here, we constructed a comprehensive rat miRNA expression B...

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Bibliographic Details
Published in:Scientific data 2022-05, Vol.9 (1), p.201-10, Article 201
Main Authors: Yao, Xintong, Sun, Shanyue, Zi, Yi, Liu, Yaqing, Yang, Jingcheng, Ren, Luyao, Chen, Guangchun, Cao, Zehui, Hou, Wanwan, Song, Yueqiang, Shang, Jun, Jiang, He, Li, Zhihui, Wang, Haiyan, Zhang, Peipei, Shi, Leming, Li, Quan-Zhen, Yu, Ying, Zheng, Yuanting
Format: Article
Language:English
Subjects:
631/337/2019
631/337/384
Animals
Data Descriptor
Developmental stages
Female
Gene Expression Profiling
Humanities and Social Sciences
Male
Medical research
MicroRNAs
MicroRNAs - genetics
miRNA
multidisciplinary
Next-generation sequencing
Rats
Rats, Inbred F344 - genetics
Reproducibility of Results
RNA, Messenger - genetics
Science
Science (multidisciplinary)
Sequence Analysis, RNA
Transcriptome
Transcriptomics
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Summary:Rat is one of the most widely-used models in chemical safety evaluation and biomedical research. However, the knowledge about its microRNA (miRNA) expression patterns across multiple organs and various developmental stages is still limited. Here, we constructed a comprehensive rat miRNA expression BodyMap using a diverse collection of 320 RNA samples from 11 organs of both sexes of juvenile, adolescent, adult and aged Fischer 344 rats with four biological replicates per group. Following the Illumina TruSeq Small RNA protocol, an average of 5.1 million 50 bp single-end reads was generated per sample, yielding a total of 1.6 billion reads. The quality of the resulting miRNA-seq data was deemed to be high from raw sequences, mapped sequences, and biological reproducibility. Importantly, aliquots of the same RNA samples have previously been used to construct the mRNA BodyMap. The currently presented miRNA-seq dataset along with the existing mRNA-seq dataset from the same RNA samples provides a unique resource for studying the expression characteristics of existing and novel miRNAs, and for integrative analysis of miRNA-mRNA interactions, thereby facilitating better utilization of rats for biomarker discovery. Measurement(s) miRNA expression profiling Technology Type(s) Next Generation Sequencing Factor Type(s) organ • age • sex Sample Characteristic - Organism Rattus norvegicus
ISSN:2052-4463
2052-4463
DOI:10.1038/s41597-022-01285-7