Defining early steps in Bacillus subtilis biofilm biosynthesis

Biofilms are the communal way of life that microbes adopt to increase survival. Key to our ability to systematically promote or ablate biofilm formation is a detailed understanding of the biofilm matrix macromolecules. Here, we identify the first two essential steps in the biofilm matrix exopolysacc...

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Bibliographic Details
Published in:mBio 2023-10, Vol.14 (5), p.e0094823
Main Authors: Arbour, Christine A, Nagar, Rupa, Bernstein, Hannah M, Ghosh, Soumi, Al-Sammarraie, Yusra, Dorfmueller, Helge C, Ferguson, Michael A J, Stanley-Wall, Nicola R, Imperiali, Barbara
Format: Article
Language:English
Subjects:
bacillosamine
biofilm
chemoenzymatic synthesis
genetic complementation
Genetics and Molecular Biology
Research Article
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Summary:Biofilms are the communal way of life that microbes adopt to increase survival. Key to our ability to systematically promote or ablate biofilm formation is a detailed understanding of the biofilm matrix macromolecules. Here, we identify the first two essential steps in the biofilm matrix exopolysaccharide (EPS) synthesis pathway. Together, our studies and approaches provide the foundation for the sequential characterization of the steps in EPS biosynthesis, using prior steps to enable chemoenzymatic synthesis of the undecaprenyl diphosphate-linked glycan substrates.
ISSN:2150-7511
2150-7511
DOI:10.1128/mbio.00948-23