Generation of induced pluripotent stem cells from large domestic animals

• Published in: Stem cell research & therapy 2020-06, Vol.11 (1), p.247-247, Article 247
• Main Authors: Bressan, Fabiana Fernandes, Bassanezze, Vinícius, de Figueiredo Pessôa, Laís Vicari, Sacramento, Chester Bittencourt, Malta, Tathiane Maistro, Kashima, Simone, Fantinato Neto, Paulo, Strefezzi, Ricardo De Francisco, Pieri, Naira Caroline Godoy, Krieger, José Eduardo, Covas, Dimas Tadeu, Meirelles, Flávio Vieira
• Format: Article
• Language: English
• Subjects: Alkaline phosphatase; Analysis; Animal behavior; Animal breeding; Animal genetic engineering; Animal models; Animal sciences; Animals; Animals, Domestic; Antibiotics; Autografts; Bovine; c-Myc protein; Cattle; Cell culture; Cell Differentiation; Cellular Reprogramming; Developmental biology; Domestic animals; Embryo cells; Embryonic Stem Cells; Equine; Fibroblasts; Horses; Induced pluripotency; Induced Pluripotent Stem Cells; iPSCs; KLF4 protein; Kruppel-Like Factor 4; Kruppel-Like Transcription Factors - genetics; Laboratory animals; Mice; Morphology; Myc protein; Oct-4 protein; Octamer Transcription Factor-3 - genetics; Phosphatase; Phosphatases; Pluripotency; SOXB1 Transcription Factors - genetics; Stem cell transplantation; Stem cells; Transcription factors; Transgenic animals; Zoology
• ISSN: 1757-6512; 1757-6512
• DOI: 10.1186/s13287-020-01716-5

Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results. Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation. Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs). The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models.