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Generation of induced pluripotent stem cells from large domestic animals
Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results. Herein, we describe the generation of ro...
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| Published in: | Stem cell research & therapy 2020-06, Vol.11 (1), p.247-247, Article 247 |
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| creator | Bressan, Fabiana Fernandes Bassanezze, Vinícius de Figueiredo Pessôa, Laís Vicari Sacramento, Chester Bittencourt Malta, Tathiane Maistro Kashima, Simone Fantinato Neto, Paulo Strefezzi, Ricardo De Francisco Pieri, Naira Caroline Godoy Krieger, José Eduardo Covas, Dimas Tadeu Meirelles, Flávio Vieira |
| description | Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results.
Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation.
Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs).
The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models. |
| doi_str_mv | 10.1186/s13287-020-01716-5 |
| format | article |
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Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation.
Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs).
The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models.</description><identifier>ISSN: 1757-6512</identifier><identifier>EISSN: 1757-6512</identifier><identifier>DOI: 10.1186/s13287-020-01716-5</identifier><identifier>PMID: 32586372</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Alkaline phosphatase ; Analysis ; Animal behavior ; Animal breeding ; Animal genetic engineering ; Animal models ; Animal sciences ; Animals ; Animals, Domestic ; Antibiotics ; Autografts ; Bovine ; c-Myc protein ; Cattle ; Cell culture ; Cell Differentiation ; Cellular Reprogramming ; Developmental biology ; Domestic animals ; Embryo cells ; Embryonic Stem Cells ; Equine ; Fibroblasts ; Horses ; Induced pluripotency ; Induced Pluripotent Stem Cells ; iPSCs ; KLF4 protein ; Kruppel-Like Factor 4 ; Kruppel-Like Transcription Factors - genetics ; Laboratory animals ; Mice ; Morphology ; Myc protein ; Oct-4 protein ; Octamer Transcription Factor-3 - genetics ; Phosphatase ; Phosphatases ; Pluripotency ; SOXB1 Transcription Factors - genetics ; Stem cell transplantation ; Stem cells ; Transcription factors ; Transgenic animals ; Zoology</subject><ispartof>Stem cell research & therapy, 2020-06, Vol.11 (1), p.247-247, Article 247</ispartof><rights>COPYRIGHT 2020 BioMed Central Ltd.</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c628t-2a612bc294ccf7603100fd81629f75c45f8c015d58c2de7cc647bb15d82ddfeb3</citedby><cites>FETCH-LOGICAL-c628t-2a612bc294ccf7603100fd81629f75c45f8c015d58c2de7cc647bb15d82ddfeb3</cites><orcidid>0000-0001-9862-5874</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318412/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2424769617?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32586372$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bressan, Fabiana Fernandes</creatorcontrib><creatorcontrib>Bassanezze, Vinícius</creatorcontrib><creatorcontrib>de Figueiredo Pessôa, Laís Vicari</creatorcontrib><creatorcontrib>Sacramento, Chester Bittencourt</creatorcontrib><creatorcontrib>Malta, Tathiane Maistro</creatorcontrib><creatorcontrib>Kashima, Simone</creatorcontrib><creatorcontrib>Fantinato Neto, Paulo</creatorcontrib><creatorcontrib>Strefezzi, Ricardo De Francisco</creatorcontrib><creatorcontrib>Pieri, Naira Caroline Godoy</creatorcontrib><creatorcontrib>Krieger, José Eduardo</creatorcontrib><creatorcontrib>Covas, Dimas Tadeu</creatorcontrib><creatorcontrib>Meirelles, Flávio Vieira</creatorcontrib><title>Generation of induced pluripotent stem cells from large domestic animals</title><title>Stem cell research & therapy</title><addtitle>Stem Cell Res Ther</addtitle><description>Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results.
Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation.
Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs).
The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models.</description><subject>Alkaline phosphatase</subject><subject>Analysis</subject><subject>Animal behavior</subject><subject>Animal breeding</subject><subject>Animal genetic engineering</subject><subject>Animal models</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Animals, Domestic</subject><subject>Antibiotics</subject><subject>Autografts</subject><subject>Bovine</subject><subject>c-Myc protein</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cellular Reprogramming</subject><subject>Developmental biology</subject><subject>Domestic animals</subject><subject>Embryo cells</subject><subject>Embryonic Stem Cells</subject><subject>Equine</subject><subject>Fibroblasts</subject><subject>Horses</subject><subject>Induced pluripotency</subject><subject>Induced Pluripotent Stem Cells</subject><subject>iPSCs</subject><subject>KLF4 protein</subject><subject>Kruppel-Like Factor 4</subject><subject>Kruppel-Like Transcription Factors - genetics</subject><subject>Laboratory animals</subject><subject>Mice</subject><subject>Morphology</subject><subject>Myc protein</subject><subject>Oct-4 protein</subject><subject>Octamer Transcription Factor-3 - genetics</subject><subject>Phosphatase</subject><subject>Phosphatases</subject><subject>Pluripotency</subject><subject>SOXB1 Transcription Factors - genetics</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Transcription factors</subject><subject>Transgenic animals</subject><subject>Zoology</subject><issn>1757-6512</issn><issn>1757-6512</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkl1r1EAUhoMotqz9A15IQBC9SJ3vmb0RStF2oSD4cT1M5mN3liSzziSi_96Tbi0bMbmY4eQ578l5eavqJUaXGCvxvmBKlGwQQQ3CEouGP6nOseSyERyTpyf3s-qilD2Ch1KEBHtenVHClaCSnFe3N37w2YwxDXUKdRzcZL2rD92U4yGNfhjrMvq-tr7rSh1y6uvO5K2vXep9GaOtzRB705UX1bMAh794OFfV908fv13fNnefbzbXV3eNFUSNDTECk9aSNbM2SIEoRig4hQVZB8kt40FZhLnjyhLnpbWCybaFgiLOBd_SVbU56rpk9vqQYXj-rZOJ-r6Q8labDP_Vec3UOhCFsCKyZYG0ignGZSslheWxM6D14ah1mNreOwvbZtMtRJdfhrjT2_RTS4oVwwQE3j4I5PRjAj90H8tslRl8moomDCtMEYeRq-r1P-g-TXkAq4AiTIq1wCfU1sACcQgJ5tpZVF-JeRfOmADq8j8UvM730abBhwj1RcO7RQMwo_81bs1Uit58_bJk35ywO2-6cVdSN80RKUuQHEGbUynZh0fjMNJzRvUxoxoyqu8zqjk0vTq1_LHlbyLpH8CA3Zg</recordid><startdate>20200625</startdate><enddate>20200625</enddate><creator>Bressan, Fabiana Fernandes</creator><creator>Bassanezze, Vinícius</creator><creator>de Figueiredo Pessôa, Laís Vicari</creator><creator>Sacramento, Chester Bittencourt</creator><creator>Malta, Tathiane Maistro</creator><creator>Kashima, Simone</creator><creator>Fantinato Neto, Paulo</creator><creator>Strefezzi, Ricardo De Francisco</creator><creator>Pieri, Naira Caroline Godoy</creator><creator>Krieger, José Eduardo</creator><creator>Covas, Dimas Tadeu</creator><creator>Meirelles, Flávio Vieira</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-9862-5874</orcidid></search><sort><creationdate>20200625</creationdate><title>Generation of induced pluripotent stem cells from large domestic animals</title><author>Bressan, Fabiana Fernandes ; Bassanezze, Vinícius ; de Figueiredo Pessôa, Laís Vicari ; Sacramento, Chester Bittencourt ; Malta, Tathiane Maistro ; Kashima, Simone ; Fantinato Neto, Paulo ; Strefezzi, Ricardo De Francisco ; Pieri, Naira Caroline Godoy ; Krieger, José Eduardo ; Covas, Dimas Tadeu ; Meirelles, Flávio Vieira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c628t-2a612bc294ccf7603100fd81629f75c45f8c015d58c2de7cc647bb15d82ddfeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alkaline phosphatase</topic><topic>Analysis</topic><topic>Animal behavior</topic><topic>Animal breeding</topic><topic>Animal genetic engineering</topic><topic>Animal models</topic><topic>Animal sciences</topic><topic>Animals</topic><topic>Animals, Domestic</topic><topic>Antibiotics</topic><topic>Autografts</topic><topic>Bovine</topic><topic>c-Myc protein</topic><topic>Cattle</topic><topic>Cell culture</topic><topic>Cell Differentiation</topic><topic>Cellular Reprogramming</topic><topic>Developmental biology</topic><topic>Domestic animals</topic><topic>Embryo cells</topic><topic>Embryonic Stem Cells</topic><topic>Equine</topic><topic>Fibroblasts</topic><topic>Horses</topic><topic>Induced pluripotency</topic><topic>Induced Pluripotent Stem Cells</topic><topic>iPSCs</topic><topic>KLF4 protein</topic><topic>Kruppel-Like Factor 4</topic><topic>Kruppel-Like Transcription Factors - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Stem cell research & therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bressan, Fabiana Fernandes</au><au>Bassanezze, Vinícius</au><au>de Figueiredo Pessôa, Laís Vicari</au><au>Sacramento, Chester Bittencourt</au><au>Malta, Tathiane Maistro</au><au>Kashima, Simone</au><au>Fantinato Neto, Paulo</au><au>Strefezzi, Ricardo De Francisco</au><au>Pieri, Naira Caroline Godoy</au><au>Krieger, José Eduardo</au><au>Covas, Dimas Tadeu</au><au>Meirelles, Flávio Vieira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of induced pluripotent stem cells from large domestic animals</atitle><jtitle>Stem cell research & therapy</jtitle><addtitle>Stem Cell Res Ther</addtitle><date>2020-06-25</date><risdate>2020</risdate><volume>11</volume><issue>1</issue><spage>247</spage><epage>247</epage><pages>247-247</pages><artnum>247</artnum><issn>1757-6512</issn><eissn>1757-6512</eissn><abstract>Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results.
Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation.
Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs).
The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>32586372</pmid><doi>10.1186/s13287-020-01716-5</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9862-5874</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1757-6512 |
| ispartof | Stem cell research & therapy, 2020-06, Vol.11 (1), p.247-247, Article 247 |
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| subjects | Alkaline phosphatase Analysis Animal behavior Animal breeding Animal genetic engineering Animal models Animal sciences Animals Animals, Domestic Antibiotics Autografts Bovine c-Myc protein Cattle Cell culture Cell Differentiation Cellular Reprogramming Developmental biology Domestic animals Embryo cells Embryonic Stem Cells Equine Fibroblasts Horses Induced pluripotency Induced Pluripotent Stem Cells iPSCs KLF4 protein Kruppel-Like Factor 4 Kruppel-Like Transcription Factors - genetics Laboratory animals Mice Morphology Myc protein Oct-4 protein Octamer Transcription Factor-3 - genetics Phosphatase Phosphatases Pluripotency SOXB1 Transcription Factors - genetics Stem cell transplantation Stem cells Transcription factors Transgenic animals Zoology |
| title | Generation of induced pluripotent stem cells from large domestic animals |
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