Long noncoding RNA transcriptome analysis reveals novel lncRNAs in Morus alba ‘Yu‐711’ response to drought stress

• Published in: The plant genome 2024-03, Vol.17 (1), p.e20273-n/a
• Main Authors: Ackah, Michael, Jin, Xin, Zhang, Qiaonan, Amoako, Frank Kwarteng, Wang, Lei, Attaribo, Thomas, Zhao, Mengdi, Yuan, Feng, Herman, Richard Ansah, Qiu, Changyu, Lin, Qiang, Yin, Zhi, Zhao, Weiguo
• Format: Article
• Language: English
• Subjects: Bioinformatics; Biosynthesis; Cellular stress response; Climate change; Cultivars; Drought; Droughts; Environmental factors; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genes; Genomes; Leaves; MicroRNAs; MicroRNAs - genetics; miRNA; Morphology; Morus; Morus alba; Non-coding RNA; Polymerase chain reaction; Proteins; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Messenger - genetics; Secondary metabolites; Transcriptomes
• ISSN: 1940-3372; 1940-3372
• DOI: 10.1002/tpg2.20273

Drought stress has been a key environmental factor affecting plant growth and development. The plant genome is capable of producing long noncoding RNAs (lncRNAs). To better understand white mulberry (Morus alba L.) drought response mechanism, we conducted a comparative transcriptome study comparing two treatments: drought‐stressed (EG) and well‐watered (CK) plants. A total of 674 differentially expressed lncRNAs (DElncRNAs) were identified. In addition, 782 differentially expressed messenger RNAs (DEmRNAs) were identified. We conducted Gene Ontology (GO) and KEGG enrichment analyses focusing on the differential lncRNAs cis‐target genes. The target genes of the DElncRNAs were most significantly involved in the biosynthesis of secondary metabolites. Gene regulatory networks of the target genes involving DElncRNAs–mRNAs–DEmRNAs and DElncRNA–miRNA–DEmRNA were constructed. In the DElncRNAs–DEmRNAs network, 30 DEmRNAs involved in the biosynthesis of secondary metabolites are collocated with 46 DElncRNAs. The interaction between DElncRNAs and candidate genes was identified using LncTar. In summary, quantitative real‐time polymerase chain reaction (qRT‐PCR) validated nine candidate genes and seven target lncRNAs including those identified by LncTar. We predicted that the DElncRNAs–DEmRNAs might recruit microRNAs (miRNAs) to interact with gene regulatory networks under the drought stress response in mulberry. The findings will contribute to our understanding of the regulatory functions of lncRNAs under drought stress and will shed new light on the mulberry–drought stress interactions. Core Ideas Mulberry response to drought stress reveals 674 differentially expressed lncRNAs. Target genes of the DElncRNAs were significantly enriched in the biosynthesis of secondary metabolites. Gene network reveals lncRNAs–miRNAs–mRNAs regulatory network involved in mulberry response to drought stress. Long noncoding RNAs participate in the host response to drought stress and interact with the target mRNAs.