Magnesium Deficiency Induced Global Transcriptome Change in Citrus sinensis Leaves Revealed by RNA-Seq

• Published in: International journal of molecular sciences 2019-06, Vol.20 (13), p.3129
• Main Authors: Yang, Lin-Tong, Zhou, Yang-Fei, Wang, Yan-Yu, Wu, Yan-Mei, Ye, Xin, Guo, Jiu-Xin, Chen, Li-Song
• Format: Article
• Language: English
• Subjects: Abscisic acid; Acidic soils; Acidification; Agricultural production; Agriculture; Allosteric properties; Antioxidants; Biological activity; Biological Transport; Biosynthesis; Calvin cycle; Carboxylation; Cell walls; cellular transport; Chlorophyll; Chloroplasts; Citrus sinensis; Citrus sinensis - genetics; Citrus sinensis - metabolism; Computational Biology - methods; Enzymes; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Ontology; Genomes; Genomics; High-Throughput Nucleotide Sequencing; Leaves; Lignin; Lipid peroxidation; Magnesium; Magnesium - metabolism; Magnesium Deficiency - genetics; Magnesium Deficiency - metabolism; Metabolism; Metabolites; Mg deficiency; Nucleic acids; Orchards; Phenotype; Physiology; Pigments; Plant Leaves - genetics; Plant Leaves - metabolism; Protein biosynthesis; Protein deficiency; Protein metabolism; Protein turnover; Proteins; Proteomics; Reactive Oxygen Species - metabolism; Ribulose-bisphosphate carboxylase; Sequence Analysis, RNA; Signal Transduction; Sucrose; Thylakoid membranes; Transcriptome
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20133129

Magnesium (Mg) deficiency is one of the major constraining factors that limit the yield and quality of agricultural products. Uniform seedlings of the were irrigated with Mg deficient (0 mM MgSO ) and Mg sufficient (1 mM MgSO ) nutrient solutions for 16 weeks. CO assimilation, starch, soluble carbohydrates, TBARS content and H O production were measured. Transcriptomic analysis of leaves was performed by Illumina sequencing. Our results showed that Mg deficiency decreased CO assimilation, but increased starch, sucrose, TBARS content and H O production in leaves. A total of 4864 genes showed differential expression in response to Mg deficiency revealed by RNA-Seq and the transcriptomic data were further validated by real-time quantitative PCR (RT-qPCR). Gene ontology (GO) enrichment analysis indicated that the mechanisms underlying Mg deficiency tolerance in may be attributed to the following aspects: a) enhanced microtubule-based movement and cell cycle regulation; b) elevated signal transduction in response to biotic and abiotic stimuli; c) alteration of biological processes by tightly controlling phosphorylation especially protein phosphorylation; d) down-regulation of light harvesting and photosynthesis due to the accumulation of carbohydrates; e) up-regulation of cell wall remodeling and antioxidant system. Our results provide a comprehensive insight into the transcriptomic profile of key components involved in the Mg deficiency tolerance in and enrich our understanding of the molecular mechanisms by which plants adapted to a Mg deficient condition.