Ciliostasis of airway epithelial cells facilitates influenza A virus infection

Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N...

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Bibliographic Details
Published in:Veterinary research (Paris) 2018-07, Vol.49 (1), p.65-65, Article 65
Main Authors: Fu, Yuguang, Tong, Jie, Meng, Fandan, Hoeltig, Doris, Liu, Guangliang, Yin, Xiangping, Herrler, Georg
Format: Article
Language:English
Subjects:
Airway systems
Cilia
Epithelial cells
Experiments
Forensic medicine
Hogs
Infections
Influenza
Influenza A virus
Influenza viruses
Life Sciences
lungs
mucus
Physiology
Short Report
sodium chloride
swine
Swine flu
Veterinary medicine
Viruses
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Summary:Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N2 subtype under ciliostatic conditions, the viral yield was about twofold or threefold higher at 24 or 48 h post-infection, respectively, as compared to slices with ciliary activity. Therefore, the cilia beating not only transports the mucus out of the airways, it also impedes virus infection.
ISSN:1297-9716
0928-4249
1297-9716
DOI:10.1186/s13567-018-0568-0