In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell

Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear. Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. B...

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Bibliographic Details
Published in:BMC complementary and alternative medicine 2012-04, Vol.12 (1), p.35-35, Article 35
Main Authors: Chong, Hueh Zan, Rahmat, Asmah, Yeap, Swee Keong, Md Akim, Abdah, Alitheen, Noorjahan Banu, Othman, Fauziah, Gwendoline-Ee, Cheng Lian
Format: Article
Language:English
Subjects:
Acanthaceae
Adenocarcinoma - drug therapy
Adenocarcinoma - metabolism
Antineoplastic Agents, Phytogenic - pharmacology
Antineoplastic Agents, Phytogenic - therapeutic use
Apoptosis
Breast Neoplasms - drug therapy
Breast Neoplasms - metabolism
Cancer
Caspase 3 - metabolism
Caspase 7 - metabolism
Cell cycle
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
Cytochromes c - metabolism
DNA Fragmentation
Female
G1 Phase - drug effects
Hormones - metabolism
Humans
Inhibitory Concentration 50
Kinases
MCF-7
Medical research
Mitochondria - drug effects
Mitochondria - metabolism
p53
Phytotherapy
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Proteins
Strobilanthes crispus
Tumor Suppressor Protein p53 - metabolism
X-Linked Inhibitor of Apoptosis Protein - metabolism
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Summary:Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear. Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC50 of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study. Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway.
ISSN:1472-6882
1472-6882
DOI:10.1186/1472-6882-12-35