Aminoguanidine affects systemic and lung inflammation induced by lipopolysaccharide in rats

• Published in: Respiratory research 2019-05, Vol.20 (1), p.96-13, Article 96
• Main Authors: Saadat, Saeideh, Beheshti, Farimah, Askari, Vahid Reza, Hosseini, Mahmoud, Mohamadian Roshan, Nema, Boskabady, Mohammad Hossein
• Format: Article
• Language: English
• Subjects: Alveoli; Aminoguanidine; Animals; Antioxidants; Antioxidants (Nutrients); Blood; Bone morphogenetic proteins; Bronchoalveolar Lavage Fluid; Bronchus; Care and treatment; Catalase; Cytokines; Diabetes; Dose-Response Relationship, Drug; Enzymes; Gene expression; Guanidines - pharmacology; Guanidines - therapeutic use; Health aspects; Immune system; Infiltration; Inflammation; Inflammation Mediators - antagonists & inhibitors; Inflammation Mediators - metabolism; Interleukin 4; Leukocytes; Levels; Lipopolysaccharide; Lipopolysaccharides; Lipopolysaccharides - toxicity; Lung diseases; Lung injury; Lungs; Lymphocytes; Macrophages; Male; Malondialdehyde; Markers; Medical research; Mitogens; Nitric oxide; Nitric-oxide synthase; Nitrites; Nitrogen oxides; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - physiology; Oxidizing agents; Pneumonia - chemically induced; Pneumonia - drug therapy; Pneumonia - metabolism; Prostaglandin E2; Prostaglandins E; Random Allocation; Rats; Rats, Wistar; Rodents; Scavenging; Studies; Superoxide dismutase; Superoxides; Thiols; Transforming growth factor-b1; Transforming growth factors; Tumor necrosis factor-TNF; White blood cell count; γ-Interferon
• ISSN: 1465-993X; 1465-9921; 1465-993X; 1465-9921
• DOI: 10.1186/s12931-019-1054-6

Nitric oxide is a mediator of potential importance in numerous physiological and inflammatory processes in the lung. Aminoguanidine (AG) has been shown to have anti-inflammation and radical scavenging properties. This study aimed to investigate the effects of AG, an iNOS inhibitor, on lipopolysaccharide (LPS)-induced systemic and lung inflammation in rats. Male Wistar rats were divided into control, LPS (1 mg/kg/day i.p.), and LPS groups treated with AG 50, 100 or 150 mg/kg/day i.p. for five weeks. Total nitrite concentration, total and differential white blood cells (WBC) count, oxidative stress markers, and the levels of IL-4, IFN-γ, TGF-β1, and PGE2 were assessed in the serum or bronchoalveolar lavage fluid (BALF). Administration of LPS decreased IL-4 level (p