Outer Membrane Proteome Analysis of Indian Strain of Pasteurella multocida Serotype B:2 by MALDI-TOF/MS Analysis

Identification of outer membrane proteins (OMPs) is important to understand the bacteria structure and function, host-pathogen interaction, development of novel vaccine candidates, and diagnostic antigens. But till now the key antigens of P. multocida B:2 isolate causing haemorrhagic septicaemia (HS...

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Bibliographic Details
Published in:TheScientificWorld 2014, Vol.2014 (2014), p.1-10
Main Authors: Singh, Vijendra Pal, Priyadarshini, Adyasha, Gupta, Santosh Kumar, Sarangi, Laxmi Narayan, Thomas, Prasad, Kumar, Santosh, Prasannavadhana, A., Nagaleekar, Viswas Konasagara
Format: Article
Language:English
Subjects:
Antigens
Bacterial infections
Bacterial Outer Membrane Proteins - immunology
Bacterial Outer Membrane Proteins - metabolism
Bacteriology
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Genomes
Gram-negative bacteria
Health aspects
Immune Sera - immunology
Immunodominant Epitopes - immunology
India
Iron
Iron - pharmacology
Membrane proteins
Methods
Molecular weight
Pasteurella multocida
Pasteurella multocida - classification
Pasteurella multocida - drug effects
Proteins
Proteome - metabolism
Proteomics - methods
Serogroup
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Studies
Time-of-flight mass spectrometry
Vaccines
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Summary:Identification of outer membrane proteins (OMPs) is important to understand the bacteria structure and function, host-pathogen interaction, development of novel vaccine candidates, and diagnostic antigens. But till now the key antigens of P. multocida B:2 isolate causing haemorrhagic septicaemia (HS) in animals are not clearly defined. In this study, P52 strain of P. multocida serotype B:2 was grown in vitro under iron-rich and iron-limited condition. The OMPs were extracted by sarkosyl method followed by SDS-PAGE and the proteins were identified by MALDI-TOF/MS analysis. In total, 22 proteins were identified, of which 7 were observed exclusively under iron-limited condition. Most of the high molecular weight proteins (TbpA, HgbA, HgbB, HasR, IroA, and HemR) identified in this study were involved in iron acquisition. Some hypothetical proteins (HP-KCU-10206, HP and AAUPMB 08244, HP AAUPMB 21592, HP AAUPMB 19766, AAUPMB 11295) were observed for the first time in this study which could be unique to serotype B:2. Further functional in vivo study of the proteins identified are required to explore the utility of these proteins in developing diagnostics and vaccine against HS.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2014/617034