Ethyl Acetate Fraction and Isolated Phenolics Derivatives from Mandevilla moricandiana Identified by UHPLC-DAD-ESI-MSn with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction

Endothelial dysfunction in obesity plays a key role in the development of cardiovascular diseases, and it is characterized by increased vascular tonus and oxidative stress. Thus, this study aimed to investigate the vasodilatory and antioxidant activities of Mandevilla moricandiana ethyl acetate frac...

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Published in:Pharmaceutics 2021-07, Vol.13 (8), p.1173
Main Authors: Ferreira, Leticia L. D. M., Leão, Valéria de F., Melo, Cinthya M. de, Machado, Thelma de B., Amaral, Ana Claudia F., Silva, Leandro L. da, Simas, Naomi K., Muzitano, Michelle F., Leal, Ivana C. R., Raimundo, Juliana M.
Format: Article
Language:English
Subjects:
Acids
Atherosclerosis
Bioavailability
Cardiovascular disease
Chromatography
Endothelium
Flavonoids
flavonols
Hypertension
Inflammation
Mandevilla moricandiana
National parks
NMR
Nuclear magnetic resonance
Obesity
Oxidative stress
Polyphenols
procyanidin
Sensors
Software
Solvents
triterpenes
vasodilation
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Summary:Endothelial dysfunction in obesity plays a key role in the development of cardiovascular diseases, and it is characterized by increased vascular tonus and oxidative stress. Thus, this study aimed to investigate the vasodilatory and antioxidant activities of Mandevilla moricandiana ethyl acetate fraction and subfractions. Vascular effects were investigated on aorta isolated from control and monosodium glutamate (MSG) induced-obese Wistar rats, and antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) methods. The ethyl acetate fraction (MMEAF) induced a concentration-dependent vasodilation on aortic rings through the NO pathway, with the involvement of histamine H1 and estrogen ERα receptors and showed potent antioxidant activity. In aorta of MSG obese rats, maximal relaxation to acetylcholine was increased in the presence of MMEAF (3 µg/mL), indicating that MMEAF ameliorated obesity-induced endothelial dysfunction. Quercetin and kaempferol aglycones and their correspondent glycosides, as well as caffeoylquinic acid derivatives, A-type procyanidin trimer, ursolic and oleanolic triterpenoid acids were identified in subfractions from MMEAF and seem to be the metabolites responsible for the vascular and antioxidant activities of this fraction.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics13081173