Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of Aggregatibacter actinomycetemcomitans

Periodontitis is a chronic inflammatory disease associated with a dysbiotic biofilm. Many pathogens have been related with its progression and severity, one of which is , a Gram-negative bacteria with seven serotypes (a-g) according with the structure of its LPS, with serotype b defined as the most...

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Bibliographic Details
Published in:Microorganisms (Basel) 2021-03, Vol.9 (3), p.622
Main Authors: Betancur, Daniel, Muñoz Grez, Camila, Oñate, Angel
Format: Article
Language:English
Subjects:
Aggregatibacter actinomycetemcomitans
Bacteria
Biofilms
Chemokines
Comparative analysis
Connective tissue
Cytokines
Diabetes
Disease
Epithelial cells
Epithelium
Gelatinase B
Gram-negative bacteria
IL-1β
Immune response
Immune system
Immunomodulation
Infections
Inflammation
Inflammatory diseases
Intercellular adhesion molecule 1
Interleukin 18
Interleukin 6
Interleukin 8
Keratinocytes
Lipopolysaccharides
Macrophages
Pathogenesis
Pathogens
Periodontitis
Reagents
Serotypes
Thymic stromal lymphopoietin
Thymus
TLR2 protein
Toll-like receptors
TRANCE protein
Tumor necrosis factor-α
Virulence
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Summary:Periodontitis is a chronic inflammatory disease associated with a dysbiotic biofilm. Many pathogens have been related with its progression and severity, one of which is , a Gram-negative bacteria with seven serotypes (a-g) according with the structure of its LPS, with serotype b defined as the most virulent compared with the other serotypes. The aim of this study was to evaluate the response of oral keratinocytes and macrophages to . Oral keratinocytes (OKF6/TERT2) and macrophages (THP-1) were infected with serotypes a, b and c. The expression of IL-1β, IL-6, IL-8, IL-18, TNF-α, MMP-9, RANKL, TLR-2, TLR-4, TLR-6, thymic stromal lymphopoietin (TSLP), and ICAM-1 was evaluated by qPCR at 2 and 24 h after infection. An increase in the expression of these molecules was induced by all serotypes at both times of infection, with macrophages showing higher levels of expression at 24 h compared to epithelial cells in which the highest levels were observed in the first hours after infection. Keratinocytes and macrophages contribute to the inflammation in periodontitis from the early stages of infection, producing the first waves of cytokines, acting as the first signal for professional immune cell recruitment and modulation of more specific immune responses.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms9030622