Association of PICK1 and BDNF variations with increased risk of methamphetamine dependence among Iranian population: a case-control study

• Published in: BMC medical genomics 2021-01, Vol.14 (1), p.27-10, Article 27
• Main Authors: Tajbakhsh, Amir, Alimardani, Maliheh, Asghari, Mahla, Abedini, Soheila, Saghafi Khadem, Sohrab, Nesaei Bajestani, Abolfazl, Alipoor, Forough, Alidoust, Maryam, Savardashtaki, Amir, Hashemian, Peyman, Pasdar, Alireza
• Format: Article
• Language: English
• Subjects: Addiction; Addictions; Adult; Amphetamine abuse; Amphetamine-Related Disorders - genetics; Binding proteins; Brain-derived neurotrophic factor; Brain-Derived Neurotrophic Factor - genetics; Carrier Proteins - genetics; Case-Control Studies; Deoxyribonucleic acid; DNA; Dopamine; Dopamine pathway; Drug abuse; Drug use; Epidemiology; Female; Gene expression; Gene polymorphism; Genetic aspects; Genetic factors; Genetic polymorphisms; Genetic Predisposition to Disease; Genetic research; Glutamate pathway; Haplotypes; Health aspects; Humans; Investigations; Iran; Kinases; Linkage disequilibrium; Male; Medical prognosis; Methamphetamine; Methamphetamine - adverse effects; Middle Aged; Nuclear Proteins - genetics; Polymorphism, Single Nucleotide; Population; Population studies; Risk factors; Single-nucleotide polymorphism; Software; Statistical analysis; Substance dependence; Substance use disorder (SUD); Systematic review
• ISSN: 1755-8794; 1755-8794
• DOI: 10.1186/s12920-021-00873-7

Genetic factors play an important role in susceptibility to methamphetamine dependency. In this line, protein that interact with C-kinase-1 (PICK1) and brain-derived neurotrophic factor (BDNF) genes are linked to methamphetamine dependence (substance use disorder). Thus, in a case-control study, we investigated the association between polymorphisms of PICK1 and BDNF genes and methamphetamine dependence in an Iranian population. Total of 235 cases and 204 controls were recruited in a period between 2015 to 2018. The PICK1-rs713729, -rs2076369 and BDNF-rs6265 genotypes were determined via ARMS-PCR assay. Statistical analysis was performed, using SPSS 20.0, PHASE 2.1.1 program as well as SNP Analyzer 2.0. In the present study, two polymorphisms including PICK1-rs713729 (OR 1.38 (CI 1.08-1.52; P- 0.004) in multiplicative and dominant models, and PICK1-rs2076369 (OR 1.31 (CI 1.10-1.56; P- 0.002) in multiplicative, dominant and co-dominant models were associated with the risk of methamphetamine abuse. Moreover, haplotype analysis showed a significant association of haplotype AG (OR 2.50 (CI 1.50-4.16; P- 0.0002) in dominant, recessive and co-dominant models, and haplotype TT (OR 0.67 (CI 0.50-0.91; P- 0.009) in dominant and co-dominant models with the risk of methamphetamine abuse. None of the polymorphisms in this study had a high level of linkage disequilibrium. Our findings indicate that the PICK1 gene polymorphism might affect the risk of methamphetamine dependency in our population.