Identification of DNA Methylation Changes in European Beech Seeds during Desiccation and Storage

Ageing and deterioration of seeds is a major problem for the maintenance of seed quality and viability during long-term storage. Prediction of early stages of seed deterioration in order to point out the plantlets' regeneration time is a major challenge of successful storage. In preserved seeds...

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Published in:International journal of molecular sciences 2023-02, Vol.24 (4), p.3557
Main Authors: Michalak, Marcin, Plitta-Michalak, Beata Patrycja, Suszka, Jan, Naskręt-Barciszewska, Mirosława Zofia, Kotlarski, Szymon, Barciszewski, Jan, Chmielarz, Paweł
Format: Article
Language:English
Subjects:
Aging
Cotyledons
Desiccation
DNA
DNA Methylation
Embryos
Epigenetics
European beech
Fagus
Fungi
Gene expression
Germination
intermediate seeds
Lipids
long-term storage
Metabolism
Methylation
Moisture content
Plantlets
Seedlings
Seeds
Seeds - genetics
Viability
Water content
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Summary:Ageing and deterioration of seeds is a major problem for the maintenance of seed quality and viability during long-term storage. Prediction of early stages of seed deterioration in order to point out the plantlets' regeneration time is a major challenge of successful storage. In preserved seeds, damages accumulate within cells at the rate mainly related to their moisture content and temperature of storage. Current research reveals global alterations in DNA methylation in lipid-rich intermediate seeds during desiccation and storage at various regimes covering nonoptimal and optimal conditions. We show for the first time that monitoring of 5-methylcytosine (m C) level in seeds can be used as a truly universal viability marker regardless of postharvest category of seeds and their composition. For seeds stored up to three years, in varied conditions, moisture content, temperature, and time of storage had significant influence on seedling emergence and DNA methylation ( < 0.05). Similarities among lipid-rich intermediate and orthodox seeds regarding different reactions of embryonic axes and cotyledons to desiccation are newly revealed. Along with previous studies on seeds dramatically different in desiccation tolerance (recalcitrant vs. orthodox), results regarding lipid-rich seeds positioned in-between (intermediate) prove that maintaining global DNA methylation status is crucial for maintaining seed viability.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24043557