Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge, a large proportion of iron in the human body is accumulated and stored in the liver. We hypothesize that increased...

Full description

Saved in:
Bibliographic Details
Published in:BMC microbiology 2020-02, Vol.20 (1), p.36-7, Article 36
Main Authors: Chen, Tao, Dong, Guofeng, Zhang, Siqin, Zhang, Xiucai, Zhao, Yajie, Cao, Jianming, Zhou, Tieli, Wu, Qing
Format: Article
Language:English
Subjects:
Abscess
Abscesses
Analysis
Animals
Bioaccumulation
Biofilm
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Cefotetan
Chelating agents
Clinical isolates
Culture Media - chemistry
Disease Models, Animal
Enterobacteriaceae
Gene expression
Gene Expression Regulation, Bacterial - drug effects
Genes
Growth
Health aspects
Humans
Imipenem
Infection
Infections
Iron
Iron - pharmacology
Iron Chelating Agents - adverse effects
Klebsiella
Klebsiella pneumoniae
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - pathogenicity
Larvae
Lepidoptera - microbiology
Liver
Liver Abscess - metabolism
Liver Abscess - microbiology
Liver diseases
Mortality
Nosocomial infections
Pneumonia
Urinary tract diseases
Urinary tract infections
Urogenital system
Virulence
Virulence (Microbiology)
Virulence - drug effects
Virulence Factors - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge, a large proportion of iron in the human body is accumulated and stored in the liver. We hypothesize that increased iron availability is an important factor driving liver abscess formation and we therefore aim to understand the effects of iron on K. pneumoniae causing liver abscesses. All tested K. pneumoniae clinical isolates, including those isolated from liver abscesses and other abdominal invasive infection sites, grew optimally when cultured in LB broth supplemented with 50 μM iron and exhibited the strongest biofilm formation ability under those conditions. Decreased growth and biofilm formation ability were observed in all tested strains when cultured with an iron chelator (P 
ISSN:1471-2180
1471-2180
DOI:10.1186/s12866-020-01727-5