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Human Hepatocyte Nuclear Factors (HNF1 and LXRb) Regulate CYP7A1 in HIV-Infected Black South African Women with Gallstone Disease: A Preliminary Study

Female sex, high estrogen levels, aging, obesity, and dyslipidemia are some of the risk factors associated with gallstone formation. HIV-infected patients on combination antiretroviral therapy (cART) are more prone to hypercholesterolemia. Bile acid synthesis is initiated by cholesterol 7-alpha hydr...

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Published in:Life (Basel, Switzerland) Switzerland), 2023-01, Vol.13 (2), p.273
Main Authors: Kinoo, Suman Mewa, Naidoo, Pragalathan, Singh, Bhugwan, Chuturgoon, Anil, Nagiah, Savania
Format: Article
Language:English
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Summary:Female sex, high estrogen levels, aging, obesity, and dyslipidemia are some of the risk factors associated with gallstone formation. HIV-infected patients on combination antiretroviral therapy (cART) are more prone to hypercholesterolemia. Bile acid synthesis is initiated by cholesterol 7-alpha hydroxylase (CYP7A1) and regulated by hepatocyte nuclear factors (HNF1α, HNF4α, and LXRb). The aim of this study was to evaluate the expression of HNF1α, HNF4α, LXRb, and miRNAs (HNF4α specific: miR-194-5p and miR-122 _1) that regulate CYP7A1 transcription in HIV-infected Black South African women on cART and presenting with gallstones relative to HIV-negative patients with gallstone disease. Females ( = 96) presenting with gallstone disease were stratified based on HIV status. The gene expression of , , , , miR-194-5p, and miR-122 _1 was determined using RT-qPCR. Messenger RNA and miRNA levels were reported as fold change expressed as 2 (RQ min; RQ max). Fold changes >2 and
ISSN:2075-1729
2075-1729
DOI:10.3390/life13020273