Aloperine Inhibits ASFV via Regulating PRLR/JAK2 Signaling Pathway In Vitro

African swine fever (ASF) has become a global pandemic due to inadequate prevention and control measures, posing a significant threat to the swine industry. Despite the approval of a single vaccine in Vietnam, no antiviral drugs against the ASF virus (ASFV) are currently available. Aloperine (ALO),...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-08, Vol.25 (16), p.9083
Main Authors: Geng, Renhao, Shao, Hongxia, Qian, Kun, Chen, Hongjun, Qin, Aijian
Format: Article
Language:English
Subjects:
African Swine Fever - metabolism
African Swine Fever - virology
African swine fever virus
African Swine Fever Virus - drug effects
African Swine Fever Virus - metabolism
aloperine
Animals
Antiviral Agents - pharmacology
Cell cycle
Cell Line
Cytokines
Cytotoxicity
Genes
Hepatitis
Hogs
Infections
JAK2 signaling pathway
Janus Kinase 2 - metabolism
Mortality
Piperidines - pharmacology
PRLR
Protein expression
Proteins
Quinolizidines - pharmacology
Signal transduction
Signal Transduction - drug effects
Swine
transcriptomics
Vaccines
Virus Replication - drug effects
Viruses
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Summary:African swine fever (ASF) has become a global pandemic due to inadequate prevention and control measures, posing a significant threat to the swine industry. Despite the approval of a single vaccine in Vietnam, no antiviral drugs against the ASF virus (ASFV) are currently available. Aloperine (ALO), a quinolizidine alkaloid extracted from the seeds and leaves of bitter beans, exhibits various biological functions, including anti-inflammatory, anti-cancer, and antiviral activities. In this study, we found that ALO could inhibit ASFV replication in MA-104, PK-15, 3D4/21, and WSL cells in a dose-dependent manner without cytotoxicity at 100 μM. Furthermore, it was verified that ALO acted on the co- and post-infection stages of ASFV by time-of-addition assay, and inhibited viral internalization rather than directly inactivating the virus. Notably, RT-qPCR analysis indicated that ALO did not exert anti-inflammatory activity during ASFV infection. Additionally, gene ontology (GO) and KEGG pathway enrichment analyses of transcriptomic data revealed that ALO could inhibit ASFV replication via the PRLR/JAK2 signaling pathway. Together, these findings suggest that ALO effectively inhibits ASFV replication in vitro and provides a potential new target for developing anti-ASFV drugs.
ISSN:1661-6596
1422-0067
1422-0067
DOI:10.3390/ijms25169083