Molecular research in the tomato breeding for resistance to biotic stressors

An urgent problem in tomato breeding is the development of universal varieties and hybrids that combine high yields and good fruit quality with group resistance to a number of diseases. The purpose of this study is to develop tomato breeding material resistant to biotic stressors, basing on modern p...

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Bibliographic Details
Main Authors: Ovod, Elena, Dubina, Elena, Garkusha, Sergey
Format: Conference Proceeding
Language:English
Subjects:
Genetic markers
Hybrids
Markers
Microsatellites
Plant breeding
Plant protection
Target recognition
Tomatoes
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Summary:An urgent problem in tomato breeding is the development of universal varieties and hybrids that combine high yields and good fruit quality with group resistance to a number of diseases. The purpose of this study is to develop tomato breeding material resistant to biotic stressors, basing on modern postgenomic technologies using molecular markers. At the initial stage, we tested microsatellite markers from foreign literature sources, the primers used in the study were synthesized by Syntol LLC, samples from the collection of FSBSI Federal Scientific Center for Biological Plant Protection and FSBSI Federal Scientific Rice Centre were studied under the conditions of Krasnodar region. PCR conditions for identifying the target Ph -3 gene in the tomato plant material used in the breeding process were optimized. Thus, in our study on the testing of microsatellite markers to identify the target Ph-3 resistance gene in the tomato genetic material, we selected 3 informative microsatellite markers R2M1S, G8-1, R1-3U, which reveal the allelic difference between susceptible and resistant samples.
ISSN:2117-4458
2273-1709
2117-4458
DOI:10.1051/bioconf/20225104004