Loading…
Chemical modification by peroxynitrite enhances TLR4 activation of the grass pollen allergen Phl p 5
The chemical modification of aeroallergens by reactive oxygen and nitrogen species (ROS/RNS) may contribute to the growing prevalence of respiratory allergies in industrialized countries. Post-translational modifications can alter the immunological properties of proteins, but the underlying mechanis...
Saved in:
Published in: | Frontiers in allergy 2023-02, Vol.4, p.1066392-1066392 |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c468t-2839656703584f1c57b66b107c642795942fec84fd431694cd76d35ac8bd5f4c3 |
---|---|
cites | cdi_FETCH-LOGICAL-c468t-2839656703584f1c57b66b107c642795942fec84fd431694cd76d35ac8bd5f4c3 |
container_end_page | 1066392 |
container_issue | |
container_start_page | 1066392 |
container_title | Frontiers in allergy |
container_volume | 4 |
creator | Reinmuth-Selzle, Kathrin Bellinghausen, Iris Leifke, Anna Lena Backes, Anna T Bothen, Nadine Ziegler, Kira Weller, Michael G Saloga, Joachim Schuppan, Detlef Lucas, Kurt Pöschl, Ulrich Fröhlich-Nowoisky, Janine |
description | The chemical modification of aeroallergens by reactive oxygen and nitrogen species (ROS/RNS) may contribute to the growing prevalence of respiratory allergies in industrialized countries. Post-translational modifications can alter the immunological properties of proteins, but the underlying mechanisms and effects are not well understood. In this study, we investigate the Toll-like receptor 4 (TLR4) activation of the major birch and grass pollen allergens Bet v 1 and Phl p 5, and how the physiological oxidant peroxynitrite (ONOO
) changes the TLR4 activation through protein nitration and the formation of protein dimers and higher oligomers. Of the two allergens, Bet v 1 exhibited no TLR4 activation, but we found TLR4 activation of Phl p 5, which increased after modification with ONOO
and may play a role in the sensitization against this grass pollen allergen. We attribute the TLR4 activation mainly to the two-domain structure of Phl p 5 which may promote TLR4 dimerization and activation. The enhanced TLR4 signaling of the modified allergen indicates that the ONOO
-induced modifications affect relevant protein-receptor interactions. This may lead to increased sensitization to the grass pollen allergen and thus contribute to the increasing prevalence of allergies in the Anthropocene, the present era of globally pervasive anthropogenic influence on the environment. |
doi_str_mv | 10.3389/falgy.2023.1066392 |
format | article |
fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_49b805f4878443f69e16c1545be20f33</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_49b805f4878443f69e16c1545be20f33</doaj_id><sourcerecordid>2783790484</sourcerecordid><originalsourceid>FETCH-LOGICAL-c468t-2839656703584f1c57b66b107c642795942fec84fd431694cd76d35ac8bd5f4c3</originalsourceid><addsrcrecordid>eNpVkc1u1DAUhSMEolXpC7BAXrKZwf8_GyQ0AlppJBAqa8txnMSVEwfbUzE7trwmT1JPZ6jahe0j33u-e6XTNG8RXBMi1YfehGG_xhCTNYKcE4VfNOeYC7LiCKKXT_RZc5nzLYQQSyglw6-bM8KlIJDK82bcjG7y1gQwxc73VRUfZ9DuweJS_L2ffUm-OODm0czWZXCz_UGBscXfHTtjD8rowJBMzmCJIbgZmHqnoYrvY_j35-9SD3vTvKorZ3d5ei-an18-32yuVttvX683n7YrS7ksKyyJ4owLSJikPbJMtJy3CArLKRaKKYp7Z2upowRxRW0neEeYsbLtWE8tuWiuj9wumlu9JD-ZtNfReP3wEdOgTSreBqepaiWsJikkpaTnyiFuEaOsdRj2hFTWxyNr2bWT66ybSzLhGfR5ZfajHuKdVkowDmkFvD8BUvy1c7noyWfrQjCzi7ussZBEqBrEoRUfW22KOSfXP45BUB8S1w-J60Pi-pR4Nb17uuCj5X--5B5ud6ns</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2783790484</pqid></control><display><type>article</type><title>Chemical modification by peroxynitrite enhances TLR4 activation of the grass pollen allergen Phl p 5</title><source>PubMed Central</source><creator>Reinmuth-Selzle, Kathrin ; Bellinghausen, Iris ; Leifke, Anna Lena ; Backes, Anna T ; Bothen, Nadine ; Ziegler, Kira ; Weller, Michael G ; Saloga, Joachim ; Schuppan, Detlef ; Lucas, Kurt ; Pöschl, Ulrich ; Fröhlich-Nowoisky, Janine</creator><creatorcontrib>Reinmuth-Selzle, Kathrin ; Bellinghausen, Iris ; Leifke, Anna Lena ; Backes, Anna T ; Bothen, Nadine ; Ziegler, Kira ; Weller, Michael G ; Saloga, Joachim ; Schuppan, Detlef ; Lucas, Kurt ; Pöschl, Ulrich ; Fröhlich-Nowoisky, Janine</creatorcontrib><description>The chemical modification of aeroallergens by reactive oxygen and nitrogen species (ROS/RNS) may contribute to the growing prevalence of respiratory allergies in industrialized countries. Post-translational modifications can alter the immunological properties of proteins, but the underlying mechanisms and effects are not well understood. In this study, we investigate the Toll-like receptor 4 (TLR4) activation of the major birch and grass pollen allergens Bet v 1 and Phl p 5, and how the physiological oxidant peroxynitrite (ONOO
) changes the TLR4 activation through protein nitration and the formation of protein dimers and higher oligomers. Of the two allergens, Bet v 1 exhibited no TLR4 activation, but we found TLR4 activation of Phl p 5, which increased after modification with ONOO
and may play a role in the sensitization against this grass pollen allergen. We attribute the TLR4 activation mainly to the two-domain structure of Phl p 5 which may promote TLR4 dimerization and activation. The enhanced TLR4 signaling of the modified allergen indicates that the ONOO
-induced modifications affect relevant protein-receptor interactions. This may lead to increased sensitization to the grass pollen allergen and thus contribute to the increasing prevalence of allergies in the Anthropocene, the present era of globally pervasive anthropogenic influence on the environment.</description><identifier>ISSN: 2673-6101</identifier><identifier>EISSN: 2673-6101</identifier><identifier>DOI: 10.3389/falgy.2023.1066392</identifier><identifier>PMID: 36873048</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>Allergy ; Bet v 1 ; dimers ; nitration ; oligomers ; Phl p 5</subject><ispartof>Frontiers in allergy, 2023-02, Vol.4, p.1066392-1066392</ispartof><rights>2023 Reinmuth-Selzle, Bellinghausen, Leifke, Backes, Bothen, Ziegler, Weller, Saloga, Schuppan, Lucas, Pöschl and Fröhlich-Nowoisky.</rights><rights>2023 Reinmuth-Selzle, Bellinghausen, Leifke, Backes, Bothen, Ziegler, Weller, Saloga, Schuppan, Lucas, Pöschl and Fröhlich-Nowoisky. 2023 Reinmuth-Selzle, Bellinghausen, Leifke, Backes, Bothen, Ziegler, Weller, Saloga, Schuppan, Lucas, Pöschl and Fröhlich-Nowoisky</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-2839656703584f1c57b66b107c642795942fec84fd431694cd76d35ac8bd5f4c3</citedby><cites>FETCH-LOGICAL-c468t-2839656703584f1c57b66b107c642795942fec84fd431694cd76d35ac8bd5f4c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975604/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975604/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36873048$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reinmuth-Selzle, Kathrin</creatorcontrib><creatorcontrib>Bellinghausen, Iris</creatorcontrib><creatorcontrib>Leifke, Anna Lena</creatorcontrib><creatorcontrib>Backes, Anna T</creatorcontrib><creatorcontrib>Bothen, Nadine</creatorcontrib><creatorcontrib>Ziegler, Kira</creatorcontrib><creatorcontrib>Weller, Michael G</creatorcontrib><creatorcontrib>Saloga, Joachim</creatorcontrib><creatorcontrib>Schuppan, Detlef</creatorcontrib><creatorcontrib>Lucas, Kurt</creatorcontrib><creatorcontrib>Pöschl, Ulrich</creatorcontrib><creatorcontrib>Fröhlich-Nowoisky, Janine</creatorcontrib><title>Chemical modification by peroxynitrite enhances TLR4 activation of the grass pollen allergen Phl p 5</title><title>Frontiers in allergy</title><addtitle>Front Allergy</addtitle><description>The chemical modification of aeroallergens by reactive oxygen and nitrogen species (ROS/RNS) may contribute to the growing prevalence of respiratory allergies in industrialized countries. Post-translational modifications can alter the immunological properties of proteins, but the underlying mechanisms and effects are not well understood. In this study, we investigate the Toll-like receptor 4 (TLR4) activation of the major birch and grass pollen allergens Bet v 1 and Phl p 5, and how the physiological oxidant peroxynitrite (ONOO
) changes the TLR4 activation through protein nitration and the formation of protein dimers and higher oligomers. Of the two allergens, Bet v 1 exhibited no TLR4 activation, but we found TLR4 activation of Phl p 5, which increased after modification with ONOO
and may play a role in the sensitization against this grass pollen allergen. We attribute the TLR4 activation mainly to the two-domain structure of Phl p 5 which may promote TLR4 dimerization and activation. The enhanced TLR4 signaling of the modified allergen indicates that the ONOO
-induced modifications affect relevant protein-receptor interactions. This may lead to increased sensitization to the grass pollen allergen and thus contribute to the increasing prevalence of allergies in the Anthropocene, the present era of globally pervasive anthropogenic influence on the environment.</description><subject>Allergy</subject><subject>Bet v 1</subject><subject>dimers</subject><subject>nitration</subject><subject>oligomers</subject><subject>Phl p 5</subject><issn>2673-6101</issn><issn>2673-6101</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkc1u1DAUhSMEolXpC7BAXrKZwf8_GyQ0AlppJBAqa8txnMSVEwfbUzE7trwmT1JPZ6jahe0j33u-e6XTNG8RXBMi1YfehGG_xhCTNYKcE4VfNOeYC7LiCKKXT_RZc5nzLYQQSyglw6-bM8KlIJDK82bcjG7y1gQwxc73VRUfZ9DuweJS_L2ffUm-OODm0czWZXCz_UGBscXfHTtjD8rowJBMzmCJIbgZmHqnoYrvY_j35-9SD3vTvKorZ3d5ei-an18-32yuVttvX683n7YrS7ksKyyJ4owLSJikPbJMtJy3CArLKRaKKYp7Z2upowRxRW0neEeYsbLtWE8tuWiuj9wumlu9JD-ZtNfReP3wEdOgTSreBqepaiWsJikkpaTnyiFuEaOsdRj2hFTWxyNr2bWT66ybSzLhGfR5ZfajHuKdVkowDmkFvD8BUvy1c7noyWfrQjCzi7ussZBEqBrEoRUfW22KOSfXP45BUB8S1w-J60Pi-pR4Nb17uuCj5X--5B5ud6ns</recordid><startdate>20230215</startdate><enddate>20230215</enddate><creator>Reinmuth-Selzle, Kathrin</creator><creator>Bellinghausen, Iris</creator><creator>Leifke, Anna Lena</creator><creator>Backes, Anna T</creator><creator>Bothen, Nadine</creator><creator>Ziegler, Kira</creator><creator>Weller, Michael G</creator><creator>Saloga, Joachim</creator><creator>Schuppan, Detlef</creator><creator>Lucas, Kurt</creator><creator>Pöschl, Ulrich</creator><creator>Fröhlich-Nowoisky, Janine</creator><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20230215</creationdate><title>Chemical modification by peroxynitrite enhances TLR4 activation of the grass pollen allergen Phl p 5</title><author>Reinmuth-Selzle, Kathrin ; Bellinghausen, Iris ; Leifke, Anna Lena ; Backes, Anna T ; Bothen, Nadine ; Ziegler, Kira ; Weller, Michael G ; Saloga, Joachim ; Schuppan, Detlef ; Lucas, Kurt ; Pöschl, Ulrich ; Fröhlich-Nowoisky, Janine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-2839656703584f1c57b66b107c642795942fec84fd431694cd76d35ac8bd5f4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Allergy</topic><topic>Bet v 1</topic><topic>dimers</topic><topic>nitration</topic><topic>oligomers</topic><topic>Phl p 5</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reinmuth-Selzle, Kathrin</creatorcontrib><creatorcontrib>Bellinghausen, Iris</creatorcontrib><creatorcontrib>Leifke, Anna Lena</creatorcontrib><creatorcontrib>Backes, Anna T</creatorcontrib><creatorcontrib>Bothen, Nadine</creatorcontrib><creatorcontrib>Ziegler, Kira</creatorcontrib><creatorcontrib>Weller, Michael G</creatorcontrib><creatorcontrib>Saloga, Joachim</creatorcontrib><creatorcontrib>Schuppan, Detlef</creatorcontrib><creatorcontrib>Lucas, Kurt</creatorcontrib><creatorcontrib>Pöschl, Ulrich</creatorcontrib><creatorcontrib>Fröhlich-Nowoisky, Janine</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Frontiers in allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reinmuth-Selzle, Kathrin</au><au>Bellinghausen, Iris</au><au>Leifke, Anna Lena</au><au>Backes, Anna T</au><au>Bothen, Nadine</au><au>Ziegler, Kira</au><au>Weller, Michael G</au><au>Saloga, Joachim</au><au>Schuppan, Detlef</au><au>Lucas, Kurt</au><au>Pöschl, Ulrich</au><au>Fröhlich-Nowoisky, Janine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical modification by peroxynitrite enhances TLR4 activation of the grass pollen allergen Phl p 5</atitle><jtitle>Frontiers in allergy</jtitle><addtitle>Front Allergy</addtitle><date>2023-02-15</date><risdate>2023</risdate><volume>4</volume><spage>1066392</spage><epage>1066392</epage><pages>1066392-1066392</pages><issn>2673-6101</issn><eissn>2673-6101</eissn><abstract>The chemical modification of aeroallergens by reactive oxygen and nitrogen species (ROS/RNS) may contribute to the growing prevalence of respiratory allergies in industrialized countries. Post-translational modifications can alter the immunological properties of proteins, but the underlying mechanisms and effects are not well understood. In this study, we investigate the Toll-like receptor 4 (TLR4) activation of the major birch and grass pollen allergens Bet v 1 and Phl p 5, and how the physiological oxidant peroxynitrite (ONOO
) changes the TLR4 activation through protein nitration and the formation of protein dimers and higher oligomers. Of the two allergens, Bet v 1 exhibited no TLR4 activation, but we found TLR4 activation of Phl p 5, which increased after modification with ONOO
and may play a role in the sensitization against this grass pollen allergen. We attribute the TLR4 activation mainly to the two-domain structure of Phl p 5 which may promote TLR4 dimerization and activation. The enhanced TLR4 signaling of the modified allergen indicates that the ONOO
-induced modifications affect relevant protein-receptor interactions. This may lead to increased sensitization to the grass pollen allergen and thus contribute to the increasing prevalence of allergies in the Anthropocene, the present era of globally pervasive anthropogenic influence on the environment.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>36873048</pmid><doi>10.3389/falgy.2023.1066392</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2673-6101 |
ispartof | Frontiers in allergy, 2023-02, Vol.4, p.1066392-1066392 |
issn | 2673-6101 2673-6101 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_49b805f4878443f69e16c1545be20f33 |
source | PubMed Central |
subjects | Allergy Bet v 1 dimers nitration oligomers Phl p 5 |
title | Chemical modification by peroxynitrite enhances TLR4 activation of the grass pollen allergen Phl p 5 |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T21%3A23%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chemical%20modification%20by%20peroxynitrite%20enhances%20TLR4%20activation%20of%20the%20grass%20pollen%20allergen%20Phl%E2%80%89p%E2%80%895&rft.jtitle=Frontiers%20in%20allergy&rft.au=Reinmuth-Selzle,%20Kathrin&rft.date=2023-02-15&rft.volume=4&rft.spage=1066392&rft.epage=1066392&rft.pages=1066392-1066392&rft.issn=2673-6101&rft.eissn=2673-6101&rft_id=info:doi/10.3389/falgy.2023.1066392&rft_dat=%3Cproquest_doaj_%3E2783790484%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c468t-2839656703584f1c57b66b107c642795942fec84fd431694cd76d35ac8bd5f4c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2783790484&rft_id=info:pmid/36873048&rfr_iscdi=true |