ASC and SVF Cells Synergistically Induce Neovascularization in Ischemic Hindlimb Following Cotransplantation

Previously, we reported the angio-vasculogenic properties of human stromal vascular fraction (SVF) and adipose tissue-derived mesenchymal stem cells (ASCs). In this study, we investigated whether the combination of ASCs and SVF cells exhibited synergistic angiogenic properties. We conducted quantita...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-12, Vol.23 (1), p.185
Main Authors: Zhang, Hong Zhe, Chae, Dong-Sik, Kim, Sung-Whan
Format: Article
Language:English
Subjects:
Adipose tissue
Angiogenesis
Animals
Apoptosis
Body fat
Cardiac function
Cell adhesion & migration
Cell Proliferation
Cell Transplantation
cotransplantation
Culture Media, Conditioned
Cytokines
Disease Models, Animal
Endothelial cells
Endothelial Cells - physiology
Fibroblast growth factor 2
Growth factors
Hindlimb - blood supply
Ischemia
Ischemia - therapy
ischemic hindlimb
Male
Mesenchymal Stem Cells
Mesenchyme
Mice
Neovascularization, Physiologic
Perfusion
Platelet-derived growth factor
Polymerase chain reaction
Stem cell transplantation
Stem cells
Stromal Vascular Fraction
Transplantation
Vascular endothelial growth factor
Vascularization
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Summary:Previously, we reported the angio-vasculogenic properties of human stromal vascular fraction (SVF) and adipose tissue-derived mesenchymal stem cells (ASCs). In this study, we investigated whether the combination of ASCs and SVF cells exhibited synergistic angiogenic properties. We conducted quantitative (q)RT-PCR, Matrigel plug, tube formation assays, and in vivo therapeutic assays using an ischemic hind limb mouse model. Immunohistochemical analysis was also conducted. qRT-PCR results revealed that FGF-2 was highly upregulated in ASCs compared with SVF, while PDGF-b and VEGF-A were highly upregulated in SVF. Conditioned medium from mixed cultures of ASCs and SVF (A+S) cells showed higher Matrigel tube formation and endothelial cell proliferation in vitro. A+S cell transplantation into ischemic mouse hind limbs strongly prevented limb loss and augmented blood perfusion compared with SVF cell transplantation. Transplanted A+S cells also showed high capillary density, cell proliferation, angiogenic cytokines, and anti-apoptotic potential in vivo compared with transplanted SVF. Our data indicate that A+S cell transplantation results in synergistic angiogenic therapeutic effects. Accordingly, A+S cell injection could be an alternative therapeutic strategy for treating ischemic diseases.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23010185