Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan

Mosquito midgut microbiota is a key component of vector competence, as gut bacteria can disturb pathogen development. In this study, we addressed the microbiota composition of Aedes aegypti during its lifespan, under field conditions. We also investigated the possible effects of environment, dietary...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2016-09, Vol.111 (9), p.577-587
Main Authors: David, Mariana Rocha, Santos, Lilha Maria Barbosa Dos, Vicente, Ana Carolina Paulo, Maciel-de-Freitas, Rafael
Format: Article
Language:English
Subjects:
Aedes
Aedes - microbiology
Animals
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Diet
diversity
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Gastrointestinal Microbiome
Insect Vectors - microbiology
mark-release-recapture
microbiota
Microbiota - physiology
PARASITOLOGY
TROPICAL MEDICINE
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Summary:Mosquito midgut microbiota is a key component of vector competence, as gut bacteria can disturb pathogen development. In this study, we addressed the microbiota composition of Aedes aegypti during its lifespan, under field conditions. We also investigated the possible effects of environment, dietary regime and ageing on the gut community composition. We employed culture independent and dependent approaches to characterise vector microbiota. There was evidence of a lifelong stable core microbiota after mosquitoes were released into an urban settlement, where they presumably fed on a range of vertebrate hosts and carbohydrate sources. This core was formed mainly of bacteria belonging to the genera Pseudomonas, Acinetobacter, Aeromonas and Stenotrophomonas and to the families Oxalobacteraceae, Enterobacteriaceae and Comamonadaceae. We showed that both dietary regime and age were associated with the abundance of some bacterial groups in the Ae. aegypti microbiota. The majority of the bacterial groups we identified have been detected in the midgut of Ae. aegypti from laboratory and wild populations, indicating a possible core microbiota associated with this mosquito species. Our findings suggest that Ae. aegypti harbours a stable bacterial community during its adult life, similar to mosquito populations from distinct geographic areas, which may be further explored for arbovirus biocontrol strategies.
ISSN:0074-0276
1678-8060
1678-8060
DOI:10.1590/0074-02760160238